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METHOD FOR PRODUCING HAPLOID, DOUBLED HAPLOID AND/OR DIHAPLOID PLANTS BY GYNOGENESIS

机译:通过生殖发生生产半周,双半周和/或双周生植物的方法

摘要

The invention relates to a method for producing haploid H, doubled haploid HD and/or dihaploid DH plants, the HD and DH being homozygous or essentially homozygous, this method being a method such as those which come under the technique of gynogenesis induced by irradiated pollen. This method comprises a step of irradiating the reproductive material of the male parent at a dose of between 160 and 190 gamma ray and/or a step of selecting the haploid H and/or DH plants by using one or more molecular marker(s). The invention also relates to a method for producing homozygous haploid, doubled haploid and/or dihaploid plants, comprising a step of determining the appropriate irradiation dose(s) for increasing the yields of said plants according to multiple given factors such as the plant species, the genotypes of the male parent and of the female parent, the climatic and weather conditions, the time at which the fruits are harvested, the level of growth of the embryos collected with a view to the culturing thereof, the level of development of the embryos placed in culture. Moreover, the invention concerns the molecular marker(s) used in the selection step and also the haploid embryos and the dihaploid embryos obtained by means of the method of the invention, and the progeny and the seeds of the plants obtained by means of the method of the invention.
机译:本发明涉及产生单倍体H,双倍单倍体HD和/或双单倍体DH植物的方法,该HD和DH是纯合的或基本上纯合的,该方法是诸如那些受辐射花粉诱导的雌激素发育的那些方法。 。该方法包括以160至190伽马射线的剂量辐照雄性亲本的生殖材料的步骤和/或通过使用一种或多种分子标记选择单倍体H和/或DH植物的步骤。本发明还涉及产生纯合单倍体,双倍单倍体和/或双单倍体植物的方法,其包括根据多种给定因素例如植物种类确定合适的辐照剂量以增加所述植物的产量的步骤。雄性母本和雌性母本的基因型,气候和天气条件,收获水果的时间,为培养目的而收集的胚的生长水平,胚的发育水平置于文化中。此外,本发明涉及在选择步骤中使用的分子标记,以及通过本发明的方法获得的单倍体胚胎和双单倍体胚胎,以及通过该方法获得的植物的后代和种子。本发明。

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