首页> 外国专利> RECOMBINANT ESCHERICHIA COLI FOR PRODUCING RECOMBINANT HUMAN VARIATION INTERFERON-BETA PROTEINS WHEREIN METHIONINE AT THE AMINO END IS REMOVED, AND PREPARATION METHOD THEREOF

RECOMBINANT ESCHERICHIA COLI FOR PRODUCING RECOMBINANT HUMAN VARIATION INTERFERON-BETA PROTEINS WHEREIN METHIONINE AT THE AMINO END IS REMOVED, AND PREPARATION METHOD THEREOF

机译:用于去除人末端蛋氨酸蛋氨酸的重组人变异干扰素-BET蛋白的重组埃希氏菌属及其制备方法

摘要

The present invention relates to recombinant Escherichia coli for producing recombinant human variation interferon-beta proteins wherein methionine at the amino end is removed, and to a preparation method thereof, and more particularly, to recombinant Escherichia coli for producing recombinant human variation interferon-beta proteins and to a preparation method thereof, wherein methionine at the amino end is removed by inserting recombinant human variation interferon-beta (rHu IFN-ßSer17, IFN-ß 1b) genes into pET21a(+) vector to produce recombinant expression vector pET21a(+)IFN-ßSer17, inserting methionine aminopeptidase genes into pSTV29 vector to produce recombinant expression vector pSTV29Map, and transforming the two recombinant expression vectors in a single colon bacillus (BL21(DE3)).
机译:本发明涉及用于生产重组人变异干扰素β蛋白的重组大肠杆菌,其制备方法,更具体地,涉及用于生产重组人变异干扰素β蛋白的重组大肠杆菌。及其制备方法,其中通过将重组人变异干扰素-β(rHuIFN-ßSer17,IFN-ß1b)基因插入pET21a(+)载体中以除去氨基末端的蛋氨酸以产生重组表达载体pET21a(+) IFN-βSer17,将甲硫氨酸氨肽酶基因插入pSTV29载体以产生重组表达载体pSTV29Map,并将两个重组表达载体转化为单个结肠杆菌(BL21(DE3))。

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