首页> 外国专利> SPECIFIC METHOD FOR THE DETECTION AND IDENTIFICATION OF ACTINOBACILLUS PLEUROPNEUMONIAE.

SPECIFIC METHOD FOR THE DETECTION AND IDENTIFICATION OF ACTINOBACILLUS PLEUROPNEUMONIAE.

机译:检测和鉴定肺炎衣原体肺炎的特定方法。

摘要

The present invention describes a specific method for the detection and diagnosis of Actinobacillus pleuropneumoniae, agent causing the porcine contagious pleuropneumonia. Due to the specificity and sensitivity, the inventive method is useful for the identification and quick diagnosis of A. pleuropneumoiae. The method comprises the use of specific oligonucleotides, which amplifies, by means of a polymerase chain reaction (PCR), a gene encoding for the 24 kDa metalloprotease from A. pleuropneumoniae, which allows biovars 1, 1b and 2 to be detected in a specific form and remove false positives in tests.
机译:本发明描述了一种用于检测和诊断引起猪传染性胸膜肺炎的细菌性胸膜肺炎放线杆菌的具体方法。由于特异性和敏感性,本发明方法可用于胸膜肺炎放线杆菌的鉴定和快速诊断。该方法包括使用特定的寡核苷酸,该寡核苷酸通过聚合酶链反应(PCR)扩增编码胸膜肺炎放线杆菌的24 kDa金属蛋白酶的基因,从而可以在特定的生物变种中检测到生物变种1、1b和2。形成并消除测试中的误报。

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