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DEVELOPMENT OF NOVEL WETTABLE POWDER FORMULATION OF POCHONIA CHLAMYDOSPORIA VAR. CHLAMYDOSPORIA AS BIONEMATICIDE AND METHODS THEROF FOR SCALE-UP PRODUCTION AND DOWN-STREAM PROCESSING FOR COMMERCIAL USE
DEVELOPMENT OF NOVEL WETTABLE POWDER FORMULATION OF POCHONIA CHLAMYDOSPORIA VAR. CHLAMYDOSPORIA AS BIONEMATICIDE AND METHODS THEROF FOR SCALE-UP PRODUCTION AND DOWN-STREAM PROCESSING FOR COMMERCIAL USE
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机译：新型的可湿性粉虱的可湿性粉剂的研制。衣原体作为杀生剂，用于商业化大规模生产和下游加工的方法
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摘要
The present invention refers to the creation of mineral silicates-based amorphous formulation (wettable powder) of beneficial fungus, Pochonia chlamydosporia var. chlamydosporia strain NBAII PC55, IMTECH accession number MTCC5583, and the processes thereof for production of its chlamydospores in economically viable yields, down-stream processing, formulating the spores on specifically designed silicate minerals-mixture and packaging. The instant invention also relates to di-phasic production and the semi-synthetic media specifically designed, which achieved increased spore yield per cycle and shortened production cycle (at least 109 chlamydospores/g substrate) both in small scale and commercial scale. The instant invention refers to the set of standardized protocols and the series of processes from fungal spore inoculation, development of formulation through down-stream processing to packaging of P. chlamydosporia var. chlamydosporia strain NBAII PC55 (MTCC5583) formulation, thus partitioning the production events and facilitates mechanization, automation and commercial use. It also defines the physical parameters of the formulation of P. chlamydosporia for long shelf-life i.e., 12 months. This invention refers to the production and utilization of Pochonia chlamydosporia var. chlamydosporia strain NBAII PC55 based bio-nematicide in biological control of root-knot nematodes and cyst nematodes as an integral component of integrated nematode management and organic farming.
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