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Catalyzing transglycosylation using a recombinant host cell overexpressing uridine phosphorylase and purine nucleoside phosphorylase

机译:使用过量表达尿苷磷酸化酶和嘌呤核苷磷酸化酶的重组宿主细胞催化转糖基化

摘要

Novel strains of genetically modified prokaryotic micro-organisms capable of expressing polypeptides having the enzyme activity of the enzymes uridine phosphorylase (UdP) and purine nucleoside phosphorylase (PNP) are described; the strains in question can be used, both in the form of whole cells and in the form of crude or purified extracts, to catalyse transglycosylation reactions between a donor nucleoside and an acceptor base with particularly high yields. The associated plasmid vectors are also described.
机译:描述了能够表达具有尿苷磷酸化酶(UdP)和嘌呤核苷磷酸化酶(PNP)的酶活性的多肽的经遗传修饰的原核微生物的新型菌株;该菌株既可以全细胞形式使用,也可以以粗提物或纯化提取物形式使用,以很高的收率催化供体核苷和受体碱基之间的转糖基化反应。还描述了相关的质粒载体。

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