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Method for culturing avian spermatogonial stem cells and avian spermatogonial stem cells prepared thereby

机译:禽精原干细胞的培养方法及由此制备的禽精原干细胞

摘要

The present invention relates to a method for long-term culturing of avian spermatogonial stem cells, which comprises the steps of: (a) preparing an avian testis; (b) isolating a population of testicular cells from said avian testis; and (c) culturing said avian spermatogonial stem cells in said population of testicular cells on a feeder cell layer in a medium containing a cell growth factor, a population of avian spermatogonial stem cells and a method for producing transgenic ayes.
机译:本发明涉及一种用于鸟类精原干细胞的长期培养的方法,该方法包括以下步骤:(a)制备鸟类睾丸; (b)从所述禽睾丸中分离出一组睾丸细胞; (c)在包含细胞生长因子,禽精原干细胞群的培养基中,在饲养细胞层上的所述睾丸细胞群中培养所述禽精原干细胞,以及产生转基因ayes的方法。

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