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rBSA from K. lactis Expression, Secretion and Purification of Recombinant Bovine Serum Albumin (rBSA) from K. lactis and uses thereof

机译:乳酸克鲁维酵母中的rBSA表达,分泌和纯化来自乳酸克鲁维酵母的重组牛血清白蛋白及其用途

摘要

A recombinant BSA (rBSA) that (a) substantially lacks deoxyribonuclease activity as determined by incubating rBSA with linear DNA overnight and gel electrophoresis, (b) lacks animal viruses associated with animal-derived cell growth supplements; and (c) is capable of stabilizing DNA proteins is provided. Methods for making the rBSA and using it to stabilize enzymes are also provided.
机译:(a)基本上缺乏脱氧核糖核酸酶活性的重组BSA(rBSA),该活性是通过将rBSA与线性DNA孵育过夜并进行凝胶电泳确定的,(b)缺乏与动物衍生的细胞生长补充剂相关的动物病毒; (c)提供了能够稳定DNA蛋白质的能力。还提供了制备rBSA并将其用于稳定酶的方法。

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