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METHOD AND APPARATUS FOR MEASURING CONCENTRATION OF VITAMIN A AND BETA-CAROTENE IN BLOOD OF DOMESTIC ANIMALS

机译:测定动物体内血液中维生素A和β-胡萝卜素浓度的方法和装置

摘要

PROBLEM TO BE SOLVED: To provide a method and apparatus for measuring a concentration of vitamin A and beta-carotene in blood of domestic animals, capable of measuring the concentration of vitamin A and beta-carotene in the blood of domestic animals in situ.;SOLUTION: The method includes a step (V) of mixing a protein modified liquid, a lipid-soluble component extract, and an antioxidant agent to prepare a mixture liquid, a step (W) of mixing and stirring the mixture liquid and a whole blood of a domestic animal to prepare a stirred mixture liquid, a step (X) of extracting vitamin A and beta-carotene in blood of the domestic animal into a layer of the lipid-soluble component extract by centrifuging the stirred mixture liquid, a step (Y) of irradiating the layer of the lipid-soluble component extract with light having two specific wavelengths and measuring each absorbance in each irradiating wavelength, and a step (Z) of computing the concentration of vitamin A and beta-carotene in the whole blood of the domestic animal from the absorbance.;COPYRIGHT: (C)2011,JPO&INPIT
机译:解决的问题:提供一种测量家畜血液中维生素A和β-胡萝卜素浓度的方法和装置,能够原位测量家畜血液中维生素A和β-胡萝卜素的浓度。解决方案:该方法包括将蛋白修饰液,脂溶性成分提取物和抗氧化剂混合以制备混合液的步骤(V),将混合液和全血混合并搅拌的步骤(W)家畜制备搅拌混合物液的步骤(X):通过离心搅拌混合物液,将家畜血液中的维生素A和β-胡萝卜素提取到脂溶性成分提取物中,步骤(X) Y),用具有两个特定波长的光照射脂溶性成分提取物的层,并测量每个照射波长下的吸光度,然后计算步骤(Z),计算维生素A和β-胡萝卜素的浓度。从吸光度获得家畜的全血。;版权所有:(C)2011,JPO&INPIT

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