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Method for the analysis of t - lymphocytes by way of the t - lymphocyte receptors of an organism
Method for the analysis of t - lymphocytes by way of the t - lymphocyte receptors of an organism
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机译:通过生物体的t淋巴细胞受体分析t淋巴细胞的方法。
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摘要
The method for analyzing the activation and motivation of T cells by analysis of T lymphocyte receptors of an organism consists in the following: determination of the number of transcripts for the gene Vbeta in order to obtain first data; amplification of the region CDR3 of the T lymphocyte receptors for each of the Vbeta genes associated with the organism; separation of the different lengths of the CDR3 of the T lymphocyte receptors for each of the Vbeta genes associated with the organism and estimation of the proportion of transcripts of each length of the CDR3 in each Vbeta family in order to obtain second data; representation of the first and second data according to the Vbeta genes and the length of the regions CDR3 in a diagram with 4 variables.
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