Incubation tray for high throughput methods in the case of protein - array - incubated

• 1. 用于玻片孵育的孵育箱及其使用方法 [P] . 中国专利： CN112816683A . 2021-05-18
• 2. 一种用于生物样品的孵育洗涤处理的装置及其孵育、回收抗体方法 [P] . 中国专利： CN109719070A . 2019-05-07
• 3. Incubation tray for protein - array - incubated [P] . 德国专利： DE202007016855U1 . 2008-03-20

  机译： 蛋白质孵育盘-阵列-孵育

• 4. transfer machine for laying eggs on a floor of a chicken coop, said eggs being located in incubation trays in which they were incubated [P] . BR112019027369A2 . 2020-08-18

  机译： 用于将鸡蛋放在鸡舍地板上的传送机，所述鸡蛋位于孵化盘中，在其中进行孵化

• 5. umlegemaschine to the laying of eggs on the bottom of a poultry house in incubation trays, in which they were incubated. [P] . 德国专利： DE202018003099U1 . 2018-08-06

  机译： 用鸡蛋在孵化盘中的禽舍底部放置卵来进行孵化。

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• 6. Methods and devices for fabricating and assembling DNA and protein arrays for high-throughput analyses. [D] . Barbee, Kristopher David. 2010
• 7. Fully Automated Sample Preparation Methods for High Throughput Transcriptome Analysis by Arrays and NGS [O] . W. Keilholz, J.D. Heath, S. Kain 2011
• 8. Articles selected by Faculty of 1000: single-cell neuronal expression analysis; array analysis of chick hair cells; coiled-coil protein arrays; high-throughput identification of plasmodesmata-interacting proteins; multicompenent therapeutics [O] . 2003

• 1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 2. Drug Discovery Non-PAGE Complex Protein Expression Analysis Tutorial: A 2-D Liquid-Phase Technique for High-Throughput Analysis of Inteact Proteins [J] . Tim Barder . 生命科学仪器 . 2004,第2期
• 3. Evaluation of Available Phosphorus by Soil Test Methods in an Acidic Soil Incubated with Different Levels of Lime and Phosphorus [J] . Ashoka Sarker ,Md. Abul Kashem ,Khan Towhid Osman . 土壤科学期刊（英文） . 2014,第3期
• 4. Comparisons of Condylar Movements with the Functional Occlusal Clutch and Tray Clutch Recording Methods in CADIAX~ system [J] . Bi-jie Han ,Hong Kang ,Lai-kui Liu . 国际口腔科学杂志 . 2010,第004期
• 5. 蛋白质免疫印迹中一种经济、有效的抗体孵育方法研究 [C] . 潘传英 ,蓝贤勇 ,陈宏 . 第五次全国动物生物技术学术研讨会 . 2010
• 6. TLR2对角质形成细胞与不同皮肤癣菌共孵育时的影响研究 [A] . 董香艳 . 2013