首页> 外国专利> Perfusion bioreactor for producing and/or cultivating human or animal tissues or tissue equivalents, where the production is base on a construct cultivated in the interior, comprises a cover, and inlet and outlet for fluid nutrient medium

Perfusion bioreactor for producing and/or cultivating human or animal tissues or tissue equivalents, where the production is base on a construct cultivated in the interior, comprises a cover, and inlet and outlet for fluid nutrient medium

机译：用于生产和/或培养人或动物组织或组织等价物的灌注生物反应器，其中生产基于内部培养的构建体，包括盖和用于液体营养培养基的入口和出口

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The perfusion bioreactor for the production and/or cultivation of human or animal tissues or tissue equivalents, where the production is base on a construct cultivated in the interior, comprises a cover (1), an inlet (2) and outlet (3) for a fluid nutrient medium. The inlet is connected with a unit for producing a perfusion pressure of the nutrient medium. The interior contour of the interior area corresponds to 100% of the surface of the external contour of the tissue to be produced or tissue equavalents. The interior contour of the bioreactor is produced through covering of scaffold (4). The perfusion bioreactor for the production and/or cultivation of human or animal tissues or tissue equivalents, where the production is base on a construct cultivated in the interior, comprises a cover (1), an inlet (2) and outlet (3) for a fluid nutrient medium. The inlet is connected with a unit for producing a perfusion pressure of the nutrient medium. The interior contour of the interior area corresponds to 100% of the surface of the external contour of the tissue to be produced or tissue equivalents. The interior contour of the bioreactor is produced through covering of scaffold (4). The scaffold is formed after the defect. The construct subjected in the interior area is flow throughable by the nutrient medium. The interior area is divided into two compartments that are disposed over self through-flow circuit and are subdivided through detachable or non-detachable separation wall. The bioreactor consists of reabsorbing or not reabsorbing hollow fiber system, guiding system or scaffold structure. The distribution of the culture medium is carried out for tissue to be produced, tissue equivalents over scaffold, guiding system, hollow fiber system or by blood vessel structures produced by tissue engineering, primarily through the perfusion and secondarily through rewinding. The bioreactor is operated as a unit in connection with a self-regulatory pulsating perfusion system. A part segment consists of 50% of elastic materials. The physical pressure regime, which is producible in the interior area, corresponds to the physical pressure regime, which influences under normal or pathological conditions in the living human or animal organism on the produced tissues, tissue equivalents, blood vessels or blood vessel networks. The value of the elasticity of the material of the part segment is adjusted so that the extension of the tissue and/or tissue equivalents together with the flexibility of the cover through the perfusion pressure produced in the interior corresponds to the physiological or pathological values of the tissue compliance of the tissue to be produced. A soft-tissue or soft tissue equivalents are produced by the bioreactor. The production of the tissue equivalents is carried out through filling the hollow area with a reabsorbing carrier material that is presettled with human or animal cells such as mesenschymal precursor cells and/or endothelial cells. The particle (7) and/or endothelial cells are admitted in a fibrin matrix. A visibility disk is subjected for optical or functional monitoring directly in the elastic cover. The bioreactor is designed for unique use. Independent claims are included for: (1) a method for the production of bioreactor; (2) a method for the production of reabsorbing temporarily hollow fiber system from a mixture of reabsorbable, biocompatible materials for the provision of the cultivated tissue with oxygen and nutrient in bioreactor; (3) a hollow fiber system; and (4) a method for the production of artificial blood vessel system by tissue engineering.

机译：用于生产和/或培养人或动物组织或组织等价物的灌注生物反应器，其中的生产基于内部培养的构建体，包括盖（1），入口（2）和出口（3），用于液体营养培养基。入口与用于产生营养培养基的灌注压力的单元连接。内部区域的内部轮廓对应于待生产的组织或组织等效物的外部轮廓的表面的100％。生物反应器的内部轮廓是通过覆盖支架（4）产生的。用于生产和/或培养人或动物组织或组织等价物的灌注生物反应器，其中的生产基于内部培养的构建体，包括盖（1），入口（2）和出口（3），用于液体营养培养基。入口与用于产生营养培养基的灌注压力的单元连接。内部区域的内部轮廓对应于待生产的组织或组织等效物的外部轮廓的表面的100％。生物反应器的内部轮廓是通过覆盖支架（4）产生的。在缺陷之后形成支架。内部区域中的构建体可被营养介质流过。内部区域分为两个隔间，两个隔间布置在自流回路上方，并通过可拆卸或不可拆卸的分隔壁细分。生物反应器由可吸收或不可吸收的中空纤维系统，导向系统或支架结构组成。培养基的分配主要是通过灌注，其次是通过重绕来进行的，该介质用于待生产的组织，支架，引导系统，中空纤维系统上的组织当量或组织工程产生的血管结构。生物反应器与自我调节的脉动灌注系统结合作为一个单元运行。零件段由50％的弹性材料组成。在内部区域可产生的物理压力状态对应于物理压力状态，该物理压力状态在正常的或病理的条件下在活的人或动物有机体中影响所产生的组织，组织等价物，血管或血管网络。调节部分段的材料的弹性值，使得组织和/或组织等效物的延伸以及通过内部产生的灌注压力的覆盖物的柔韧性对应于组织的生理或病理学值。要生产的组织的组织顺应性。生物反应器产生软组织或等同的软组织。组织等同物的生产是通过在中空区域填充一种可再吸收的载体材料来实现的，该材料预先填充了人或动物细胞，例如间充质前体细胞和/或内皮细胞。颗粒（7）和/或内皮细胞被接纳在纤维蛋白基质中。可见盘直接在弹性盖中进行光学或功能监控。该生物反应器专为独特用途而设计。独立权利要求包括：（1）一种生物反应器的生产方法; （2）一种由可吸收的生物相容性材料的混合物生产暂时吸收中空纤维系统的方法，以在生物反应器中为培养的组织提供氧气和营养。（3）中空纤维系统; （4）一种通过组织工程生产人造血管系统的方法。

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公开/公告号DE102008015635A1

专利类型
公开/公告日2009-10-01

原文格式PDF
申请/专利权人 NOVA TISSUE GMBH;
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申请/专利号DE20081015635
发明设计人 FRERICH BERNHARD;
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申请日2008-03-25
分类号C12M3/04;C12M1/42;C12M1/40;
国家 DE
入库时间 2022-08-21 19:09:11

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