首页> 外国专利> METHOD FOR PRODUCING EMBRYONAL FIBROBLAST-LIKE CELLS FROM BUNNY RANGE OF A NEWBORN

METHOD FOR PRODUCING EMBRYONAL FIBROBLAST-LIKE CELLS FROM BUNNY RANGE OF A NEWBORN

机译:从新生兔宝宝范围生产胚状成纤维样细胞的方法

摘要

1. A method of producing fibroblast-like cells from the umbilical cord of a newborn, comprising preparing a fragment of the umbilical cord of a person, isolating fibroblast-like cells by treatment with a type I collagenase solution and centrifugation, characterized in that the prepared fragment of the umbilical cord is homogenized, and the homogenate is treated with a solution containing type I collagenase type IV collagenase with an enzyme ratio of 1: 1 in a proportion of homogenate to an enzyme solution of 1: 5. ! 2. The method according to claim 1, characterized in that in the process of preparing a fragment of the umbilical cord, endothelial cells are isolated from the umbilical cord vein.
机译:1.一种从新生儿的脐带产生成纤维细胞样细胞的方法,包括制备人的脐带的片段,通过用I型胶原酶溶液处理并离心分离出成纤维细胞样细胞,其特征在于:将制备的脐带片段均质化,并用含有I型胶原酶,IV型胶原酶的溶液处理匀浆,该酶的比例为匀浆与酶液的比例为1:5,酶比为1:1。 2.根据权利要求1所述的方法,其特征在于,在制备所述脐带的片段的过程中,从所述脐带静脉分离出内皮细胞。

相似文献

  • 专利
  • 外文文献
  • 中文文献
获取专利

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号