The present invention dual-line relates to a high-voltage two-dimensional liquid chromatography apparatus, and more specifically, the sample is a mixture of strong cation exchange the solvent and the analyte is introduced, mixed strong cation exchange solvent inlet port, a sample inlet port, the sample discharge port, a sample storage loop connection port and the column select the sample inlet valve including a valve connection port; Determining whether to transfer the incoming sample to the first column valve or whether the second column valve be transferred to, and a sample inlet valve connection port, the first column valve connection port and the column select valve including a second column valve connection port .; A first first-column solid phase extraction column and a first valve which is reverse-phase liquid chromatography column in fluid communication; The second valve 2 column solid phase extraction column and a second reverse-phase liquid chromatography column in fluid communication; The solvent selection valve that is one column valve and the second valve in fluid communication with the supply column using a mixed solvent of the second solvent and the first solvent or a first solvent to the first column and the second column valve valve; The first solvent and the second solvent inlet valve, which is the second solvent inlet and a solvent mixer in fluid communication; A second solvent supply pump for supplying a second solvent to the second solvent inlet valve; A first solvent supply pump for the second solvent inlet valve, and supplying a first solvent to the solvent selection valve; And a mixed strong cation exchange solvent supply pump for supplying the mixed strong cation exchange solvent to the sample inlet valve, the column selection valve connection port and said sample inlet valve connection ports double line in fluid communication by a strong cation exchange column It relates to a two-dimensional super-high pressure liquid chromatography apparatus. ; According to the invention, the steel by cation exchange chromatography and reverse phase liquid chromatography using a two-dimensional liquid chromatography apparatus connected online were maximize analysis efficiency of complex biological samples, in particular tissue paper time (dead time) to minimize, rapid injection of the sample , on-line sample desalting process and the sample concentrated implementation, as well as to achieve high repeatability of the retention time of the liquid chromatography, the double sample that does not even have been produced under different conditions lowering the efficiency of the solid-phase extraction column-line ultra-high pressure It may provide a two-dimensional liquid chromatography apparatus. ; Two-dimensional liquid chromatography, reverse phase liquid chromatography, solid phase extraction column, strong cation exchange chromatography
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