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Avirulent attenuated strain of Pasteurella multocida and live vaccine containing the same
Avirulent attenuated strain of Pasteurella multocida and live vaccine containing the same
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机译：多杀巴斯德氏菌无毒减毒株及含有该毒株的活疫苗
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摘要
An avirulent attenuated strain of Pasteurella multocida is provided to improve the prevention and treatment efficiency of swine pasteurellosis caused by Pasteurella multocida by removing PMT(Pasteurella multocida toxin) from Pasteurella multocida through knocking-out of a toxA gene of Pasteurella multocida, so that the strain is useful for producing a live vaccine. An avirulent attenuated strain of Pasteurella multocida(KCCM 10824P) wherein a toxA gene is knocked-out is produced by transforming Pasteurella multocida with a polynucleotide consisting of a 0.52 kb N-terminal fragment of Pasteurella multocida toxA gene, an antibiotic-resistant gene and a 0.44 kb C-terminal fragment of Pasteurella multocida toxA gene, and has the nucleotide sequence of SEQ ID NO:1, wherein the antibiotic is kanamycin. A live vaccine for preventing and treating swine pasteurellosis contains the avirulent attenuated strain of Pasteurella multocida(KCCM 10824P).
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机译：提供了无毒的多杀巴斯德氏菌减毒株，通过敲除多杀巴斯德氏菌的toxA基因，从多杀巴斯德氏菌中去除PMT（多杀巴斯德氏菌毒素），从而提高了由多杀巴斯德氏菌引起的猪巴氏杆菌病的预防和治疗效率。可用于生产活疫苗。通过用由多杀性巴斯德氏菌toxA基因的0.52 kb N端片段，抗生素抗性基因和p53组成的多核苷酸转化多杀性巴斯德氏菌来生产多杀巴斯德氏菌的无毒减毒株（KCCM 10824P）。多杀性巴斯德氏菌toxA基因的0.44kb C-末端片段，并具有SEQ ID NO：1的核苷酸序列，其中抗生素是卡那霉素。预防和治疗猪巴氏杆菌病的活疫苗含有无毒的多杀巴斯德氏菌减毒株（KCCM 10824P）。
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