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A PROCESS OF PREPARING ADENOSINE DEAMINASE HAVING ENHANCED ACTIVITY, A VARIANT OF ADENOSINE DEAMINASE HAVING ENHANCED ACTIVITY, AND A PROCESS OF PREPARING 2'-DEOXYGUANOSINE FROM DADPR USING THE SAME
A PROCESS OF PREPARING ADENOSINE DEAMINASE HAVING ENHANCED ACTIVITY, A VARIANT OF ADENOSINE DEAMINASE HAVING ENHANCED ACTIVITY, AND A PROCESS OF PREPARING 2'-DEOXYGUANOSINE FROM DADPR USING THE SAME
A method for increasing enzyme activity of adenosine deaminase(ADD) to 2,6-diaminopurine-2'-deoxyriboside(dDAPR) is provided to increase the enzyme activity using human derived ADD and produce deoxyguanosine with high yield. A nucleotide coding human adenosine deaminase is denoted by the sequence number 3. A transformed strain is produced by transforming the nucleotide of the sequence number 3 in E.coli BL21 strain. A method for producing dGR from the dDAPR comprises: a step of transforming the nucleotide in E.coli BL21 strain and culturing the transformed strain; and a step of converting dDAPR with dGR using adenosine deaminase from medium.
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