首页>
外国专利>
CONSTRUCTION OF THE VECTORS EXPRESSING REPORTER GENES DRIVEN BY THE ENDOTHELIAL CELL-SPECIFIC PROMOTERS AND THEIR APPLICATION IN MONITORING OF MESENCHYMAL STEM CELLS DIFFERENTIATING INTO THE ENDOTHELIAL LINEAGE
CONSTRUCTION OF THE VECTORS EXPRESSING REPORTER GENES DRIVEN BY THE ENDOTHELIAL CELL-SPECIFIC PROMOTERS AND THEIR APPLICATION IN MONITORING OF MESENCHYMAL STEM CELLS DIFFERENTIATING INTO THE ENDOTHELIAL LINEAGE
A monitoring method of the differentiation to an endothelial cell using a recombinant expression vector containing a promoter and reporter gene inducing the endothelial cell is provided to treat as a gene therapy of cardiovascular disease and stem cell therapy. A recombinant expression vector pEGFP/Flk-1 or pEGFP/Tie-2 for monitoring the differentiation of endothelial cell comprises a promoter which induces the endothelial cell-specific expression and reporter gene which is connected to be operatable. The endothelial cell-specific promoter is the Flk-1 promoter of sequence number 1 (SEQ ID NO:1) and Tie-2 promoter of sequence number 2. The reporter gene is green fluorescence protein(GFP), CFP, YFP, or DsRed. A method for monitoring the differentiation of the endothelial cell comprises: a step of preparing a mesenchymal stem cell; a step of inducing a recombinant expression vector for monitoring the differentiation of endothelial cell into the mesenchymal stem cell; and a step of confirming the expression of reporter gene in the transformed mesenchymal stem cell.
展开▼
机译：提供一种使用含有启动子和诱导内皮细胞的报告基因的重组表达载体向内皮细胞分化的监测方法，以作为心血管疾病的基因疗法和干细胞疗法。用于监测内皮细胞分化的重组表达载体pEGFP / Flk-1或pEGFP / Tie-2包含诱导内皮细胞特异性表达的启动子和连接成可操作的报道基因。内皮细胞特异性启动子是序列号1（SEQ ID NO：1）的Flk-1启动子和序列号2的Tie-2启动子。报告基因是绿色荧光蛋白（GFP），CFP，YFP或DsRed 。一种监测内皮细胞分化的方法，包括：制备间充质干细胞的步骤;和诱导重组表达载体以监测内皮细胞向间充质干细胞分化的步骤;以及确定报告基因在转化的间充质干细胞中表达的步骤。
展开▼
