首页> 外国专利> THE METHOD TO IDENTIFY CERVI PARVUM CORNU SPECIES USING DIAGNOSING OLIGONUCLEOTIDE OF CERVUS SPECIES AND A CERVI PARVUM CORNU SPECIES DIAGNOSING DNA CHIP

THE METHOD TO IDENTIFY CERVI PARVUM CORNU SPECIES USING DIAGNOSING OLIGONUCLEOTIDE OF CERVUS SPECIES AND A CERVI PARVUM CORNU SPECIES DIAGNOSING DNA CHIP

机译:利用鹿类诊断寡核苷酸和宫颈小核糖核酸诊断DNA芯片鉴定鹿小孢子的方法

摘要

A method for identifying Cervi Parvum Cornu species by using an oligonucleotide molecule is provided to conveniently and rapidly distinguish the Cervi Parvum Cornu species from the dried slice of Cervi Parvum Cornu, thereby preventing illegal distribution of other animal horns. The method for identifying Cervi Parvum Cornu species by using an oligonucleotide molecule comprises the steps of: PCR amplifying the gene of deer including ATPase8(ATP synthase subunit) or CO1(cytochrome C oxidase subunit 1) gene by using the DNA extracted from an object as a template; developing the PCR product to electrophoresis, and analyzing the nucleotide sequence of the electrophoresis product; and confirming the sequence of oligonucleotide molecule in the analyzed nucleotide sequence.
机译:本发明提供了一种通过使用寡核苷酸分子鉴定小鹿角种的方法,以方便快捷地从干燥的小鹿角片中区分小鹿角,从而防止其他动物角的非法分布。通过使用寡核苷酸分子鉴定小圆角鹿种的方法包括以下步骤:通过使用从对象提取的DNA作为载体,PCR扩增包括ATPase8(ATP合酶亚基)或CO1(细胞色素C氧化酶亚基1)基因的鹿的基因。模板开发用于电泳的PCR产物,并分析电泳产物的核苷酸序列;并确定所分析核苷酸序列中寡核苷酸分子的序列。

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