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Ex-vivo expansion method under clinical grade conditions of haematopoietic stem cells isolated from bone marow to treat ischaemic diseases such as acute myocardial infartcion
Ex-vivo expansion method under clinical grade conditions of haematopoietic stem cells isolated from bone marow to treat ischaemic diseases such as acute myocardial infartcion
This invention concerns an expansion method under clinical grade conditions of haematopoietic bone marrow CD34+ stem cells including the phases of: (i) isolation, for immunomagnetic selection, of haematopoietic stem cells from a sample of autologous bone marrow using a closed commercial system CliniMacs; (ii) exposition of haematopoietic stem cells to such conditions to permit expansion and addressing them in a mono-endothelial sense, preparation of cell therapy product resuspended in 10 ml of physiological solution and intracoronary grafting using haemodynamic and heart surgery techniques.;This invention also concerns the clinical use of haematopoietic stem cells of marrow blood obtained to treat ischaemic pathologies such as acute myocardial infarction.
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