首页> 外国专利> ANALYSIS AND TYPING OF MONOCLONAL PROTEINS BY MEANS OF CAPILLARY ELECTROPHORESIS AND IMMUNO DISTRIBUTION

ANALYSIS AND TYPING OF MONOCLONAL PROTEINS BY MEANS OF CAPILLARY ELECTROPHORESIS AND IMMUNO DISTRIBUTION

机译:毛细血管电泳和免疫分布的方法对单核蛋白的分析和分型

摘要

Analyzing a biological sample by capillary electrophoresis consists of initiating negatively charged modified antibodies so that they migrate to a zone outside the migration zone of the sample's proteins when they are separated during electrophoresis, with the antibodies having an antigen specificity for a predetermined target protein. The constituents of a first aliquot part of the biological sample are separated by capillary electrophoresis in the presence of the negatively charged antibodies capable of forming an antigen-antibody complex with a protein that might be present in the sample, and the separated constituents are detected. The electrophoretic profile obtained in the first stage is compared with the profile of the constituents of another aliquot part of the same sample separated by capillary electrophoresis in the absence of the modified antibodies.
机译:通过毛细管电泳分析生物样品包括启动带负电荷的修饰抗体,以使它们在电泳过程中分离时会迁移到样品蛋白质迁移区域之外的区域,并且抗体对预定的目标蛋白质具有抗原特异性。在能够与样品中可能存在的蛋白质形成抗原-抗体复合物的带负电荷的抗体的存在下,通过毛细管电泳分离生物样品的第一等分部分的成分,并且检测分离的成分。将第一阶段获得的电泳图谱与在没有修饰抗体的情况下通过毛细管电泳分离的同一样品的另一等分部分的成分图谱进行​​比较。

著录项

  • 公开/公告号AT418735T

    专利类型

  • 公开/公告日2009-01-15

    原文格式PDF

  • 申请/专利权人 SEBIA;

    申请/专利号AT20040292651T

  • 发明设计人 ROBERT FREDERIC;ANDRE REGIS;

    申请日2004-11-09

  • 分类号G01N33/537;G01N33/561;C07K16;C07K16/06;C07K16/18;G01N27/447;G01N33/68;

  • 国家 AT

  • 入库时间 2022-08-21 19:24:39

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