首页> 外国专利> IMMUNOGENIC COMPOSITIONS COMPRISING VENEZUELAN EQUINE ENCEPHALITIS VIRUS REPLICON VECTORS AND PARAMYXOVIRUS PROTEIN ANTIGENS

IMMUNOGENIC COMPOSITIONS COMPRISING VENEZUELAN EQUINE ENCEPHALITIS VIRUS REPLICON VECTORS AND PARAMYXOVIRUS PROTEIN ANTIGENS

机译:包括委内瑞拉马脑中的脑炎病毒复制子载体和肺炎病毒蛋白质抗原的免疫原性成分

摘要

Self-propagating, fusogenic blebs are produced from cells infected with a population of Venezuelan Equine Encephalitis virus replicon particles (VRP). The self-propagating, fusogenic nature of the blebs is derived from expression of heterologous genes encoding viral fusion proteins that are incorporated into the replication defective replicon particles. The resulting blebs can be harvested from supernatants of cells displaying severe cytopathic effects. The blebs are used to make immunogenic compositions and devise methods of immunizing mammals against paramyxoviruses such as parainfluenza virus type 3.
机译:自蔓延融合的气泡是由感染了委内瑞拉马脑炎病毒复制子颗粒(VRP)的细胞产生的。气泡的自我传播,融合性质来自编码整合到复制缺陷复制子颗粒中的病毒融合蛋白的异源基因的表达。可以从显示出严重细胞病变作用的细胞上清液中收获产生的气泡。气泡用于制备免疫原性组合物,并设计出针对副粘病毒(如副流感病毒3型)免疫哺乳动物的方法。

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