首页> 外国专利> Particle based homogeneous assays using capillary electrophoresis with laser-induced fluorescence detection

Particle based homogeneous assays using capillary electrophoresis with laser-induced fluorescence detection

机译:基于颗粒的均相测定,使用毛细管电泳和激光诱导的荧光检测

摘要

The invention provides highly sensitive and rapid homogeneous assays which employ particle-enhanced assay formats in concert with capillary electrophoresis and laser-induced fluorescence (LIF) detection to determine the concentration of an analyte of interest in a sample. Such a determination is made by measuring fluorescent signal(s) (i.e., an electropherogram) produced upon LIF of species present in the reaction mixture that are capable of producing such signals. The method of this invention produces simplified electropherograms by reducing the number of signals that must be separated and subsequently measured, and therefore increases the accuracy of the detection and/or quantification of target analyte concentration in a sample.
机译:本发明提供了高灵敏度和快速的均相测定法,其采用与毛细管电泳和激光诱导的荧光(LIF)检测相结合的颗粒增强的测定形式来确定样品中目标分析物的浓度。这种确定是通过测量LIF反应混合物中存在的能够产生这种信号的物质而产生的荧光信号(即电泳图)来进行的。本发明的方法通过减少必须分离并随后测量的信号的数量来产生简化的电泳图,并因此提高了样品中目标分析物浓度的检测和/或定量的准确性。

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