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DNA dependent protein kinase catalytic subunit phosphorylation sites and antibodies thereto

机译:DNA依赖性蛋白激酶催化亚基磷酸化位点及其抗体

摘要

The identification and use of two major DNA-PKcs autophosphorylation sites. Threonine (T) 2609 and Serine (S) 2056, including antibodies specific for phosphorylated T2609 and 52056. Peptides and polynucleotides encoding same, that feature these two sites of phosphorylation. The antibodies do not bind to the unphosphorylated DNA-PKcs protein or peptide, thus providing diagnostic tools to monitor the effectiveness of treatments which target the DNA repair pathway of cancer cells, and the ability to intervene or inhibit in phosphorylation of T2609 or 52056, either through application of a drug or an antibody, to increase the radiosensitivity of cancer cells.
机译:鉴定和使用两个主要的DNA-PKcs自磷酸化位点。苏氨酸(T)2609和丝氨酸(S)2056,包括特异于磷酸化T2609和52056的抗体。具有这两个磷酸化位点的肽和编码它们的多核苷酸。抗体不与未磷酸化的DNA-PKcs蛋白或肽结合,因此提供了诊断工具来监控靶向癌细胞DNA修复途径的治疗的有效性以及干预或抑制T2609或52056磷酸化的能力通过应用药物或抗体来增加癌细胞的放射敏感性。

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