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Regulation of gene expression using chromatin remodelling factors

机译:使用染色质重塑因子调控基因表达

摘要

The invention provides a method to regulate expression of a gene of interest in a plant comprising, introducing into the plant a first nucleotide sequence comprising, the gene of interest operatively linked to a first regulatory region, and an operator sequence capable of binding a fusion protein, and a second nucleotide sequence comprising a second regulatory region in operative association with a nucleotide sequence encoding the fusion protein. The fusion protein comprising, a DNA binding protein, or a portion thereof, capable of binding the operator sequence, and a recruitment factor protein, or a portion thereof, capable of binding a chromatin remodelling protein. In this manner, expression of the second nucleotide sequence produces the fusion protein that regulates expression of the gene of interest.
机译:本发明提供了一种在植物中调节目的基因表达的方法,该方法包括将第一核苷酸序列引入该植物,该第一核苷酸序列包含与第一调节区可操作地连接的目的基因,以及能够结合融合蛋白的操纵子序列。 ;和第二核苷酸序列,其包含与编码融合蛋白的核苷酸序列可操作地缔合的第二调节区。所述融合蛋白包含能够结合操纵子序列的DNA结合蛋白或其一部分,以及能够结合染色质重塑蛋白的募集因子蛋白或其一部分。以这种方式,第二核苷酸序列的表达产生了调节目的基因表达的融合蛋白。

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