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Identification and application of genes and their products that promote hybrid stress or hybrid weakness

机译:鉴定和应用能促进杂种优势或杂种优势的基因及其产物

摘要

The present invention relates to a method of identifying candidate genes and the proteins encoded by them that are useful in the inducement of hybrid vigour, hybrid debility and/or the diagnosis, prognosis and treatment of disease. In particular, the present invention relates to a method for identifying candidate genes capable of producing hybrid vigour or hybrid debility in an animal or plant, comprising the steps of: (i) comparing the mRNA sequence of alleles of candidate genes isolated from an animal or plant which exhibits hybrid vigour or hybrid debility with the nucleotide sequences from the corresponding alleles isolated from the parents of said animal or plant; (ii) identifying mRNA sequence differences in the alleles from said animal or plant which exhibits hybrid vigour or hybrid debility which codes for amino acid sequence variation; and (iii) identifying that the amino acid sequence variation between alleles of the candidate gene in said animal or plant is encoded by mRNA sequences which are located within two or more different exons within the candidate gene.
机译:本发明涉及鉴定候选基因和由其编码的蛋白质的方法,所述候选基因和由其编码的蛋白质可用于诱导杂种优势,杂种缺陷和/或疾病的诊断,预后和治疗。特别地,本发明涉及用于鉴定能够在动植物中产生杂种活力或杂种缺陷的候选基因的方法,其包括以下步骤:(i)比较从动物或动物中分离的候选基因的等位基因的mRNA序列。与从所述动物或植物的亲本分离的相应等位基因的核苷酸序列表现出杂种活力或杂种缺陷的植物; (ii)鉴定来自所述动物或植物的等位基因中的mRNA序列差异,其表现出编码氨基酸序列变化的杂种活力或杂种缺陷性; (iii)鉴定所述动物或植物中候选基因的等位基因之间的氨基酸序列变异是由位于候选基因内两个或更多个不同外显子内的mRNA序列编码的。

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