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Strategies that have been improved for sequencing complex genomes using high-throughput sequencing technology
Strategies that have been improved for sequencing complex genomes using high-throughput sequencing technology
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机译：使用高通量测序技术对复杂基因组进行测序的策略已得到改进
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摘要
A step of digesting the genome with the restriction endonuclease of the first one, and a step of ligating the restriction fragments of the first subset, the adapter ligated restriction fragments using a combination of the first primer, at least one adapter least A step of selectively amplifying the first set of, the first primer, containing (including selective nucleotides 1-10) select the first sequence to the 3 'end of the primer sequence, a step of amplifying, comprising the steps of repeating these processes by using a combination of the second primer at least the steps of containing a selected sequence of different second repeat, primers, adapters ligated restriction fragments are amplified A process of fragmenting each subset, and there the step of generating a sequencing library thereby fragmenting, and determining steps the nucleotide sequence of the fragment, in the step of aligning the sequences of the fragments in the library, respectively Te, the method comprising: generating contigs thereby aligning, a step of repeating these steps for further restriction endonuclease and / or a second restriction endonuclease one further restriction endonucleases, and / or a second restriction endonuclease the method comprising aligning the contigs obtained for each nuclease, a step of providing a sequence of the genome thereby align, method for determining a genome sequence.
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