首页> 外国专利> Being the contentious nuclear acid fragment which contentious nuclear acid fragment, the kit for fixed quantity of rearrangement body gene, joins one which at least

Being the contentious nuclear acid fragment which contentious nuclear acid fragment, the kit for fixed quantity of rearrangement body gene, joins one which at least

机译:作为有争议的核酸片段,有争议的核酸片段,固定数量的重排体基因的试剂盒,至少连接一个

摘要

As for this invention, the competitive nuclear acid fragment which is used for ideal for competitive PCR method, the kit for fixed quantity of gene recombination body gene, quantitative method of the gene recombination body gene due to competitive PCR method is offered.The competitive nuclear acid fragment which connects one which at least corresponds to the unique gene in the rearrangement gene part of one which corresponds to the indwelling characteristic gene part of the gene recombination body gene 1st competitive nuclear acid molecule and the gene recombination body gene 2nd competitive nuclear acid molecule at least, arranges on identical nuclear acid; The kit in order the indwelling characteristic gene of the said competitive nuclear acid fragment and the gene recombination body gene and includes with the 1st primer opposite in order to expand the 1st competitive nuclear acid molecule, and the rearrangement gene of the gene recombination body gene and the 2nd primer opposite in order the 2nd competitive nuclear acid molecule to expand to use for the fixed quantity of the gene recombination body gene; And using the said competitive nuclear acid fragment or the said kit, it features that it does competitive PCR method, quantitative method of the gene recombination body gene is offered.
机译:对于本发明,提供了用于竞争性PCR方法的理想的竞争性核酸片段,固定数量的基因重组体基因的试剂盒,由于竞争性PCR方法而产生的基因重组体基因的定量方法。酸片段,其连接至少一个对应于基因重组体基因第一竞争性核酸分子和基因重组体基因第二竞争性核酸分子的留置特征基因部分的独特基因的重排基因部分至少排列在相同的核酸上;该试剂盒是为了使上述竞争性核酸片段和基因重组体基因的残存特征基因与基因重组体基因相对应而设置的,并具有与第一引物相反的引物,用于扩增第一竞争性核酸分子,以及该基因重组体基因的重排基因。第二引物相对于第二竞争核酸分子以用于定量固定的基因重组体基因。并且,使用上述竞争性核酸片段或上述试剂盒,具有进行竞争性PCR法,提供基因重组体基因的定量方法的特征。

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