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A biochemical method and apparatus for the determination of properties of proteins

机译:测定蛋白质性质的生化方法和装置

摘要

There is described a biochemical method for detecting one or more protein characteristics. The method utilizes supports (1), wherein the largest dimension (3) of each support (1) is less than 250 νm and wherein each support (1) incorporates sequential identification means (2). The method is distinguished in that it includes the steps of: attaching an information molecule (7), which is capable of interacting with at least one of said one or more protein characteristic to be detected, to a main surface (11) of a support (1); suspending supports (1) comprising one or more different sequential identifications means (2) and one or more different information molecules (7) in a fluid; adding a sample (8) to be analysed to the fluid; detecting interaction signals from supports (1) in the fluid using signal detecting means (40); and reading the sequential identification means (2) of the supports (1) which have an interaction signal using reading means (3), thereby detecting at least one of said one or more protein characteristic (8). There is also described apparatus susceptible for use in executing the above method.
机译:描述了一种用于检测一种或多种蛋白质特征的生化方法。该方法利用支架(1),其中每个支架(1)的最大尺寸(3)小于250μm,并且其中每个支架(1)结合有顺序识别装置(2)。该方法的特征在于其包括以下步骤:将能够与所述一种或多种待检测的蛋白质特征中的至少一种相互作用的信息分子(7)附着到支持物的主表面(11)上。 (1);将包含一种或多种不同的顺序识别工具(2)和一种或多种不同的信息分子(7)的支持物(1)悬浮在流体中;将要分析的样品(8)添加到流体中;使用信号检测装置(40)检测来自流体中的载体(1)的相互作用信号;使用读取装置(3)读取具有相互作用信号的支持物(1)的顺序识别装置(2),从而检测所述一种或多种蛋白质特征(8)中的至少一种。还描述了易于在执行上述方法中使用的设备。

著录项

  • 公开/公告号DE60219429T2

    专利类型

  • 公开/公告日2008-01-03

    原文格式PDF

  • 申请/专利权人

    申请/专利号DE2002619429T

  • 发明设计人

    申请日2002-02-13

  • 分类号G01N33/543;

  • 国家 DE

  • 入库时间 2022-08-21 19:47:56

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