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Producing biofilm causing biogenic sulfuric acid corrosion on surface of test body arranged in reactor container of bioreactor, comprises inoculating test body with microorganism and releasing hydrogen sulfide gas flow in reactor container
Producing biofilm causing biogenic sulfuric acid corrosion on surface of test body arranged in reactor container of bioreactor, comprises inoculating test body with microorganism and releasing hydrogen sulfide gas flow in reactor container
The method for producing biofilm (2) causing biogenic sulfuric acid corrosion on a surface of a test body (4) arranged in a reactor container (3) of a bioreactor (1), comprises inoculating the test body with microorganisms of the type of Thiobacillus and if necessary with other microorganisms and releasing a hydrogen sulfide gas flow in the reactor container to produce a hydrogen sulfide containing gas atmosphere in the surrounding region of the test body. The test body is inserted into the measuring unit. The test bodies are arranged in the reactor container. The method for producing biofilm (2) causing biogenic sulfuric acid corrosion on a surface of a test body (4) arranged in a reactor container (3) of a bioreactor (1), comprises inoculating the test body with microorganisms of the type of Thiobacillus and if necessary with other microorganisms and releasing a hydrogen sulfide gas flow in the reactor container to produce a hydrogen sulfide containing gas atmosphere in the surrounding region of the test body. The test body is inserted in the measuring unit. The test bodies are arranged in the reactor container. The hydrogen sulfide gas flow is led to each surface of the test bodies and is fed from the outside of the reactor container and/or the hydrogen sulfide gas flow is produced in the reactor container. The hydrogen sulfide concentration in a gas chamber of the reactor container partially surrounding the test body is adjusted to a value of = 100 ppm. The reactor container is partially filled with an aqueous solution (7), which is heated at a given temperature of 35[deg] C, is mixed with a nutrient solution and is inoculated with the microorganisms. The test body for inoculating the microorganisms is sprayed with the aqueous solution having the microorganisms. The test body is arranged in the reactor container, so that a part of the surface of the test body dips in the liquid phase. The hydrogen sulfide gas flow is released in the region below from a liquid level of the liquid phase. The test body is sprayed once with the aqueous solution at an interval of 24 hours. The part of the surface of the test body arranged in the gas chamber is partially sprayed out by gas pressure surges from the aqueous solution. The gas pressure surges are caused by supply of the hydrogen sulfide gas flow in the liquid phase. The pH-value of the aqueous solution is adjusted to a value of 1.0-2.0. An independent claim is included for a bioreactor for producing biofilm causing biogenic sulfuric acid corrosion on a surface of a test body.
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