首页> 外国专利> Modular system for functionalization of sensor surface in medical analytics with biological active substance for producing DNA chip and micro arrays, comprises metallic surface on which nucleic acid and/or its derivatives are immobilized

Modular system for functionalization of sensor surface in medical analytics with biological active substance for producing DNA chip and micro arrays, comprises metallic surface on which nucleic acid and/or its derivatives are immobilized

机译：用于具有生物活性物质的医学分析中的传感器表面功能化的模块化系统，该生物活性物质用于生产DNA芯片和微阵列，该系统包括固定了核酸和/或其衍生物的金属表面

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摘要

The modular system for the functionalization of implant surface or sensor surface in medical, biological, molecular genetic and biochemical analytics with biological active substance for the production of DNA chip and micro arrays, comprises a metallic surface (2) on which nucleic acid and/or its derivatives (4) are immobilized. The metallic surface is provided with a metal oxide layer (3). The nucleic acid and/or its derivatives carry on its 5' or 3' terminal anionic group (5), which forms within the metal oxide layer by anchor strand (7). The modular system for the functionalization of implant surface or sensor surface in medical, biological, molecular genetic and biochemical analytics with biological active substance for the production of DNA chip and micro arrays, comprises a metallic surface (2) on which nucleic acid and/or its derivatives (4) are immobilized. The metallic surface is provided with a metal oxide layer (3). The nucleic acid and/or its derivatives carry on its 5' or 3' terminal anionic group (5), which forms within the metal oxide layer by anchor strand (7). A complementary stabilization strand is bound on each anchor strand in stabilization area. The anchor strand with the stabilization strand is fixed as a helical double strand in the metal oxide layer, while the functionalization surface turned to the metal oxide layer is formed single strand. The anchor strand and the stabilization strand have anionic group on both single stranded terminals of nucleic acid and/or its derivatives. The functionalization exists in a hybridization, by which the complementary nucleic acid strand is bound on the single stranded parts of the anchor strand at the functionalization area through complimentary base pairing. Active agents such as inorganic, organic, biochemical molecule and/or cellular fabric components are bound on the complementary nucleic acid. The strand immobilized on the metal surface and the complementary strands are covalently connected with one another. An independent claim is included for a procedure for the production of modular system for the immobilization of nucleic acid and/or its derivatives on metallic surface.

机译：用于在医学，生物，分子遗传和生化分析中使用生物活性物质对植入物表面或传感器表面进行功能化的模块化系统，用于生产DNA芯片和微阵列，该系统包括一个金属表面（2），在该金属表面上核酸和/或其衍生物（4）被固定。金属表面设有金属氧化物层（3）。核酸和/或其衍生物带有其5'或3'末端阴离子基团（5），该阴离子基团通过锚链（7）在金属氧化物层内形成。用于在医学，生物，分子遗传和生化分析中使用生物活性物质对植入物表面或传感器表面进行功能化的模块化系统，用于生产DNA芯片和微阵列，该系统包括一个金属表面（2），在该金属表面上核酸和/或其衍生物（4）被固定。金属表面设有金属氧化物层（3）。核酸和/或其衍生物带有其5'或3'末端阴离子基团（5），该阴离子基团通过锚链（7）在金属氧化物层内形成。互补稳定链束缚在稳定区域中的每个锚链上。具有稳定链的锚链在金属氧化物层中被固定为螺旋双链，而转向金属氧化物层的官能化表面形成为单链。锚链和稳定链在核酸和/或其衍生物的两个单链末端上均具有阴离子基团。功能化存在于杂交中，通过该杂交，互补核酸链通过互补碱基配对在功能化区域处结合在锚链的单链部分上。诸如无机，有机，生化分子和/或细胞织物成分的活性剂结合在互补核酸上。固定在金属表面上的链和互补链彼此共价连接。包括用于将核酸和/或其衍生物固定在金属表面上的模块化系统的生产方法的独立权利要求。

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公开/公告号DE102006024058A1

专利类型
公开/公告日2007-11-22

原文格式PDF
申请/专利权人 TECHNISCHE UNIVERSITAET DRESDEN;
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申请/专利号DE20061024058
发明设计人 ISRAEL INA;SCHWENZER BERND;SCHARNWEBER DIETER;BEUTNER RENE;MICHAEL JAN;
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申请日2006-05-16
分类号C12Q1/68;A61L27/54;
国家 DE
入库时间 2022-08-21 19:49:55

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