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METHOD FOR ANTI-INFLAMMATORY ACTIVITY DETERMINATION OF IMMUNOGLOBULINS INTRAVENOUS PREPARATIONS

机译:免疫球蛋白静脉注射制剂的抗炎活性测定方法

摘要

FIELD: medicine.;SUBSTANCE: invention represents method for anti-inflammatory activity determination of immunoglobulins intravenous (IGIV) preparations. To biological liquor sample, obtained from acute aseptic inflammation focus and including "acute-inflammation" polymorphonuclear phagocytes, immunoglobulins intravenous preparation is added in quantity of 50% of reaction medium volume, intensity of spontaneous luminol-dependent chemiluminescence is determined, and light sums difference of chemiluminescence responce, compared to check sample, is calculated. Decrease in chemiluminescence light sum in the presence of IGIV preparation by 50% and more indicates anti-inflammatory activity.;EFFECT: invention allows determine suppressing action level of IGIV preparations, aiding in treatment preparations selection for cases with negative manifestations of local and system inflammatory process.;2 ex, 2 tbl
机译:发明领域:本发明代表用于测定免疫球蛋白静脉内(IGIV)制剂的抗炎活性的方法。对于从急性无菌炎症灶获得的包括“急性炎症”多形核吞噬细胞的生物液样品,以反应培养基体积的50%的量添加免疫球蛋白静脉内制剂,确定自发性鲁米诺依赖性化学发光的强度,并且求和的总和不同与对照样品相比,计算了化学发光响应的百分比。在存在IGIV制剂的情况下化学发光光总量减少50%以上表明抗炎活性。效果:本发明可以确定IGIV制剂的抑制作用水平,有助于局部和系统炎症阴性的病例的治疗制剂的选择。过程。; 2 ex,2 tbl

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