首页> 外国专利> METHOD FOR SPECIES IDENTIFICATION OF MYCOBACTERIUM OF MAIS COMPLEX (M. AVIUM, M. INTRACELLULARE, M. SCROFULACEUM) AND M. TUBERCULOSIS

METHOD FOR SPECIES IDENTIFICATION OF MYCOBACTERIUM OF MAIS COMPLEX (M. AVIUM, M. INTRACELLULARE, M. SCROFULACEUM) AND M. TUBERCULOSIS

机译：麦芽孢杆菌（M. Avium，M。内部脑膜炎，M。SCROFULACEUM）和结核分枝杆菌的菌种鉴定的方法

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FIELD: medicine, analytical biochemistry, microbiology.;SUBSTANCE: method involves isolation of DNA by heating mycobacteria at 95^°C for 12 min in TE-buffer, pH 8.0. Prepared amplicons are treated with restrictase Hin 6I (volume of amplicons for restriction reaction is 17 mcl, restriction time is 1 h 10 min). Products of restriction are separated in agarose gel followed by analysis of restriction patterns of species to be studied. Invention provides increasing safety of DNA and to obtain the distinct picture of restriction analysis.;EFFECT: improved method for identification of mycobacteria.;2 cl, 1 dwg

机译：领域：方法：包括通过在95℃的pH值为8.0的TE缓冲液中加热分枝杆菌12分钟来分离DNA的方法。制备的扩增子用限制酶Hin 6I处理（用于限制反应的扩增子量为17 mcl，限制时间为1 h 10分钟）。在琼脂糖凝胶中分离限制性产物，然后分析待研究物种的限制性模式。发明提供了增加的DNA安全性并获得了限制性分析的清晰图片。效果：鉴定分枝杆菌的改进方法; 2 cl，1 dwg

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公开/公告号RU2319748C2

专利类型
公开/公告日2008-03-20

原文格式PDF
申请/专利权人 MOSKOVSKIJ GORODSKOJ NAUCHNO-PRAKTICHESKIJ TSENTRBORBY S TUBERKULEZOM;
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申请/专利号RU20040137089
发明设计人 GALKINA KSENIJA JUREVNA;NOSOVA ELENA JUREVNA;MOROZ ARKADIJ MAKSOVICH;LITVINOV VITALIJ ILICH;KRASNOVA MARIJA ALEKSANDROVNA;SKOTNIKOVA OLGA IVANOVNA;
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申请日2004-12-20
分类号C12Q1/68;
国家 RU
入库时间 2022-08-21 19:50:44

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