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首页> 外国专利> BACTERIOPHAGE N15 REPLICON-BASE VECTOR AND RECOMBINANT VECTOR FOR REGULATED EXPRESSION OF TARGET GENE IN ESCHERICHIA COLI CELLS, STRAIN ESCHERICHIA COLI PROVIDING POSSIBILITY FOR REGULATION OF VECTOR COPY NUMBER AND EXPRESSION SYSTEM

BACTERIOPHAGE N15 REPLICON-BASE VECTOR AND RECOMBINANT VECTOR FOR REGULATED EXPRESSION OF TARGET GENE IN ESCHERICHIA COLI CELLS, STRAIN ESCHERICHIA COLI PROVIDING POSSIBILITY FOR REGULATION OF VECTOR COPY NUMBER AND EXPRESSION SYSTEM

机译：噬菌体N15复制子矢量和重组矢量可调节大肠埃希氏菌细胞中靶基因的表达，应变大肠埃希氏菌提供了调节矢量拷贝数和表达系统的可能性

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FIELD: biotechnology, molecular biology, genetic engineering.;SUBSTANCE: invention relates to creature of the expression system of target proteins in Escherichia coli cells that combines high level of biosynthesis of the end product and large range of its regulation. Invention proposes the constructed expression systems representing strain E. coli comprising phage N15 anti-repressor gene integrated into chromosome of cells wherein this gene is under control of functionally active promoter induced in E. coli, and sop-operon of phage N15 transformed with recombinant vector based on phage N15 replicon and comprising DNA fragment encoding the end protein under control of promoter in chromosome or another induced promoter. Proposed vectors are inherited stably in growing microorganisms in antibiotic-free medium based on the correct distribution of replicated plasmid copies between daughter cells. Using the invention provides the broad range of regulation of the yield of the end product and possibility for using a single expression system for preparing the large assortment of protein products being independently of their functional activity.;EFFECT: valuable biological properties of vector.;20 cl, 7 dwg, 2 tbl, 8 ex
机译：技术领域：本发明涉及大肠杆菌细胞中靶蛋白表达系统的生物，其结合了高水平的终产物的生物合成和大范围的调节。本发明提出了构建的表达系统，其代表大肠杆菌菌株，其包含整合到细胞染色体中的噬菌体N15抗阻遏物基因，其中该基因在大肠杆菌中诱导的功能活性启动子的控制下，以及用重组载体转化的噬菌体N15的Sop操纵子该酶基于噬菌体N15复制子，并包含编码在染色体启动子或另一种诱导启动子控制下的末端蛋白的DNA片段。根据复制的质粒拷贝在子细胞之间的正确分布，建议的载体在无抗生素培养基中的生长微生物中稳定遗传。使用本发明提供了调节终产物产率的广泛范围，并提供了使用单一表达系统来制备种类繁多的蛋白质产物的可能性，而与它们的功能活性无关。效果：载体的宝贵生物学特性; 20 cl，7 dwg，2 tbl，8 ex

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