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Makers for the diagnosis of susceptibility to lung cancer using survivin gene and method for predicting and analyzing susceptibility to lung cancer using the same
Makers for the diagnosis of susceptibility to lung cancer using survivin gene and method for predicting and analyzing susceptibility to lung cancer using the same
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机译：利用Survivin基因诊断肺癌易感性的制造商以及使用该基因的预测和分析肺癌易感性的方法
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摘要
A marker for diagnosing lung cancer, a kit and a microarray therefor, and a method for predicting and determining susceptibility to lung cancer are provided to find out the possibility of being endangered by the lung cancer by sorting out genetic disposition based on polymorphism of a survivin gene, thereby being usefully used for predicting and determining the lung cancer. A marker for diagnosing susceptibility to lung cancer consists of at least one polynucleotide selected from the group consisting of a polynucleotide which consists of 20-100 DNA sequences having a 479th base of SEQ ID : NO. 1(-31st base from a translation starting point of a survivin gene), the 479th base G being substituted with C, a polynucleotide which consists of 20-100 DNA sequences having a 343rd base of SEQ ID : NO. 3(9194th gene from the translation starting point of the survivin gene), the 343rd base G being substituted with A, and a complimentary sequence thereof. A kit for diagnosing susceptibility to lung cancer comprises a primer amplifying the polynucleotide including the 479th base of the SEQ ID : NO. 1 or the 343rd base of the SEQ ID : NO. 3, wherein the primer is at least one primer selected from the group consisting of SEQ ID : NOs. 7 to 8, SEQ ID : NOs. 15 to 16, and SEQ ID : NOs. 23 to 29. A microarray for diagnosing lung cancer comprises at least one of the polynucleotides. A method for predicting and determining susceptibility to lung cancer comprises the steps of: (a) obtaining a nucleic acid sample from a test body; (b) amplifying at least one polymorphism selected from the group consisting of the 479th base of the SEQ ID : NO. 1(-31st base from the translation starting point of the survivin gene), a 125th base of SEQ ID : NO. 2(-625th base from the translation starting point of the survivin gene), the 343rd base of the SEQ ID : NO. 3(9194th base from the translation starting point of the survivin gene), and a 298th base of SEQ ID : NO. 4(9809th base from the translation starting point of the survivin gene); and (c) determining the base of the amplified polymorphism portion to analyze the danger of the lung cancer generation.
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机译：提供了用于诊断肺癌的标记物，用于其的试剂盒和微阵列，以及用于预测和确定对肺癌的易感性的方法，以通过基于存活蛋白的多态性挑选出遗传处置来发现被肺癌危害的可能性。基因，因此可用于预测和确定肺癌。用于诊断对肺癌的易感性的标记物包括至少一种选自多核苷酸的多核苷酸，该多核苷酸由具有SEQ ID NO：4的第479个碱基的20-100个DNA序列组成。 1（从survivin基因的翻译起始点起-31个碱基），第479个碱基G被C取代，C是由20-100个具有SEQ ID NO：3的第343个碱基的DNA序列组成的多核苷酸。 3（从survivin基因的翻译起始点起第9194个基因），第343个碱基G被A及其互补序列取代。诊断肺癌易感性的试剂盒包括引物，该引物扩增包含SEQ ID NO：4的第479个碱基的多核苷酸。 SEQ ID NO：1或第343位碱基。参照图3，其中引物是选自SEQ ID NO：3的至少一种引物。 SEQ ID NO：7至8。 SEQ ID NO：15至16。 23-29。用于诊断肺癌的微阵列，其包含至少一种多核苷酸。一种预测和确定对肺癌易感性的方法，包括以下步骤：（a）从测试体获得核酸样品; （b）扩增选自SEQ ID NO：4的第479个碱基的至少一种多态性。 SEQ ID NO：1（从survivin基因的翻译起始点起-31个碱基），第125个碱基。 SEQ ID NO：2（从survivin基因的翻译起始点起第-625个碱基），第343个碱基。 3（从survivin基因的翻译起点起9194个碱基）和SEQ ID NO：2的第298个碱基。 4（从survivin基因的翻译起点算起的第9809个碱基）; （c）确定扩增的多态性部分的碱基以分析产生肺癌的危险。
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