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The preparing method of inactivated vaccine of Swine Erysipelas prepared with inactivated antigen and specific purified protein antigen of Swine Erysipelas
The preparing method of inactivated vaccine of Swine Erysipelas prepared with inactivated antigen and specific purified protein antigen of Swine Erysipelas
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机译：用猪丹毒杆菌的灭活抗原和特异纯化蛋白抗原制备的猪丹毒的灭活疫苗的制备方法
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摘要
A swine erysipelas inactivated vaccine is provided to significantly improve the immune capacity by adding a protein antigen obtained from a culture supernatant to a swine erysipelas inactivated antigen. The swine erysipelas inactivated vaccine is prepared by the method comprising the steps of: (a) after inoculating swine erysipelas seed into a swine erysipelas producing culture medium, culturing it at a temperature of 37 deg.C for 18-20 hours and adding formaline thereto to inactivate it, centrifuging it with the speed of 6,000 rpm for 30 minutes and collecting supernatant after removing settled down bacteria therefrom; (b) gradually adding ammonium sulfate to the supernatant until the solution being saturated to separate protein therefrom; (c) after putting the protein obtained from the step (b) in a dialysis membrane to remove impurities therefrom and obtain purified protein; (d) floating the purified protein in PBS(pH of 7.2) to prepare a swine erysipelas specific antigen; and (e) adding the swine erysipelas specific antigen to a swine erysipelas inactivated antigen to have 10-50% of the swine erysipelas specific antigen therein and mixing it. The swine erysipelas inactivated vaccine further comprises an immunostimulant.
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