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DEVELOPMENTS OF AN AGAR DIFFUSION METHOD TO MEASURE ELASTEASE INHIBITION ACTIVITY USING ELASTIN-CONGO RED
DEVELOPMENTS OF AN AGAR DIFFUSION METHOD TO MEASURE ELASTEASE INHIBITION ACTIVITY USING ELASTIN-CONGO RED
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机译：弹性蛋白-刚果红在琼脂扩散法中测定弹性蛋白酶抑制活性的研究进展
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摘要
A method for measuring elastase inhibition activity is provided to screen, identify and quantify an elastase inhibitor in a sample through a simple method of putting an agar medium under constant temperature condition without requiring a separate measuring instrument. A method for measuring elastase inhibition activity comprises the steps of: (a) preparing a solid culture medium using agar medium including 0.01-1.0 wt.% of Elastin-Congo red; (b) after adding a sample which is subject to elastase inhibition activity measurement to the solid culture medium and putting it in an incubator under constant temperature condition of 30-42 deg.C for 3-72 hours, measuring a diameter of halo shown on the culture medium.
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