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FUNCTION-MODIFIED PHENYLALANINE DEHYDROGENASE, AND METHOD FOR ANALYSIS OF AMINO ACID IN BIOLOGICAL SAMPLE USING THE ENZYME
FUNCTION-MODIFIED PHENYLALANINE DEHYDROGENASE, AND METHOD FOR ANALYSIS OF AMINO ACID IN BIOLOGICAL SAMPLE USING THE ENZYME
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机译：功能修饰的苯丙氨酸脱氢酶及利用酶法分析生物样品中氨基酸的方法
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摘要
A modified enzyme having such modification of at least three amino acid residues that the modified enzyme can have improved substrate specificity of phenylalanine dehydrogenase (EC 1.4.1.20) for methionine; DNA encoding the modified enzyme; a method for the preparation of a modified enzyme which has such modification of at least three amino acid residues that the modified enzyme can have improved substrate specificity of phenylalanine dehydrogenase (EC 1.4.1.20) for methionine, comprising the steps of culturing a transformant transformed with a vector carrying the DNA, and collecting the modified enzyme from the culture; and a method for the analysis of L-methionine contained in a sample by using the modified enzyme or protein. A phenylalanine dehydrogenase having a substrate specificity for L-methionine can be produced by an evolutionary molecular engineering approach to provide a function-modified amino acid dehydrogenase suitable for enzymatic fluorometry of methionine. It becomes possible to provide a method for the analysis of L-methionine contained in a sample (a blood sample) by using the function-modified amino acid dehydrogenase.
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