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ENZYME SUBSTRATES, CULTURE MEDIA CONTAINING SAME AND USE THEREOF IN ORDER TO DETECT AMINOPEPTIDASE ACTIVITY AND/OR DIFFERENTIATE GRAM-POSITIVE BACTERIA FROM GRAM-NEGATIVE BACTERIA
ENZYME SUBSTRATES, CULTURE MEDIA CONTAINING SAME AND USE THEREOF IN ORDER TO DETECT AMINOPEPTIDASE ACTIVITY AND/OR DIFFERENTIATE GRAM-POSITIVE BACTERIA FROM GRAM-NEGATIVE BACTERIA
The invention relates to novel chromogenic enzyme substrates which are used to detect aminopeptidase activity in micro-organisms or to determine whether at least one bacterium belongs to the Gram-positive group or the Gram-negative group according to the colour thereof. The invention also relates to culture media containing such substrates, the use of said substrates or media for the detection of aminopeptidase activities and/or the differentiation of Gram-positive bacteria in relation to Gram-negative bacteria and use methods thereof. The aforementioned novel substrates have the following formula, wherein: R1 is nothing or an alkyl, allyl, aryl group; R2 is formed by at least one amino acid, preferably alanine; R3, R4, R5 and R6 are formed independently of one another by H- or -O-alkyl, preferably -O-CH3; R7 is formed by H, O-CH3, alkyl or halogen; R8 is formed by H or Cl; and n is an integer corresponding to 0 or 1. The invention is particularly suitable for use in the field of diagnostics.
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机译:本发明涉及新颖的生色酶底物,其用于检测微生物中的氨肽酶活性或根据其颜色确定至少一种细菌是革兰氏阳性基团还是革兰氏阴性基团。本发明还涉及含有这种底物的培养基,所述底物或培养基在检测氨肽酶活性和/或相对于革兰氏阴性细菌的革兰氏阳性细菌的分化中的用途及其使用方法。上述新型底物具有下式:其中R1为空或烷基,烯丙基,芳基; R 2由至少一种氨基酸,优选丙氨酸形成; R3,R4,R5和R6彼此独立地由H-或-O-烷基,优选-O-CH3形成; R7由H,O-CH3,烷基或卤素形成; R8由H或Cl形成; n是0或1的整数。本发明特别适用于诊断领域。
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