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METHOD FOR EXTRACTING α-SPECIFIC TROMBIN-LIKE ENZYME (ANCISTRON-B) FROM AGKISTRODON BLOMHOFFII USSURIENSIS VENOM
METHOD FOR EXTRACTING α-SPECIFIC TROMBIN-LIKE ENZYME (ANCISTRON-B) FROM AGKISTRODON BLOMHOFFII USSURIENSIS VENOM
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机译：牛AG乌头蛇毒提取α-特异凝血酶样酶（ANCISTRON-B）的方法。
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摘要
The invention relates to producing bioactive substances and can be used for biotechnological processes, in medicine and in the pharmacological industry, in particular to producing medicinal and diagnosis preparations from snake venom. The inventive method consists in carrying out affinity chromatography using a sodium chloride step gradient, wherein, at the first stage, 10mM Tris HCl with a pH of 7.4 is used in the form of a buffer solution and said solution having a sodium chloride concentration of 2M is used in the form of an eluent. The albumin peak exhibiting trombin-like activity is collected, concentrated and, with a view to substitutea buffer, is applied to a column with Sephadex G 25 which is balanced by a 50mM Tris HCl buffer with a pH of 7.4 and a 0.13 M NaCl content.
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机译：本发明涉及生产生物活性物质，并且可以用于生物技术过程，在医学和药物工业中，特别是从蛇毒生产药物和诊断制剂。本发明的方法包括使用氯化钠逐步梯度进行亲和层析，其中在第一步中，以缓冲溶液的形式使用pH为7.4的10mM Tris HCl，所述溶液的氯化钠浓度为2M。以洗脱液的形式使用。收集，浓缩表现出类似于类胰蛋白活性的白蛋白峰，并考虑使用替代缓冲液，将其上样至Sephadex G 25色谱柱上，该色谱柱由50mM Tris HCl缓冲液平衡，pH 7.4且NaCl含量为0.13 M 。
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