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A METHOD FOR IDENTITY TESTING BY PROBE ELONGATION-MEDIATED ANALYSIS OF VARIABLE SITES IN THE HLA GENE IN A GENOME
A METHOD FOR IDENTITY TESTING BY PROBE ELONGATION-MEDIATED ANALYSIS OF VARIABLE SITES IN THE HLA GENE IN A GENOME
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机译：基因组中HLA基因可变位点的探针延伸介导分析的身份测试方法
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摘要
The present invention is related to a method for identity testing by probe elongation-mediated analysis of variable sites in the HLA gene in a genome by providing sets of different cognate probes, the cognate probes in a set capable of annealing to one or more amplicons, said amplicons generated by amplifying regions of the genome using asymmetric polymerase chain reaction, and said cognate probes further capable of being elongated with a detectably labeled nucleotide if, following annealing, the probe's interrogation site is complementary to the aligned nucleotide in the amplicon, wherein said probes are designed such that the aligned nucleotide is complementary to a nucleotide at a variable site; and designating for each set of amplicons, one strand (either sense or antisense) for the probe elongation-mediated analysis of the variable sites, depending on which strand has a greater degree of complementarity to its cognate probe in the terminal elongation initiation region of the cognate probe.
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