The invention relates to a process for the isolation of novel compound 8-(Cβ-D- glucopyranosyl )-7, 3",4"- trihydroxyflavone from Pterocarpus marsupium. The process steps are: a)powdering of the heartwood of the plant Pterocarpus marsupium, b) extracting the powdered plant material so prepared, with protic solvent, such as herein described, c) concentrating the extract to minimum volume and partitioning with different organic solvents such as herein described of increasing polarity to remove non polar components, extracting the aqueous layer with polar solvent, removing the solvent to get residue, d) isolating the 8-(C-β-D-glucopyranosyl)-7,3",4"-trihydroxyflavone from the residue by conventional chromatographic methods.
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