首页> 外国专利> Methods of preparing a matrix chromatographic polissacaru00ecdeo cross-linked to provide a substantially sterile chromatographic column and to provide a membrane polissacaru00ecdeo substantially sterile chromatographic matrix porous polissacaru00ecdeo cross-linked chromatographic column. The use of a chromatographic matrix.

Methods of preparing a matrix chromatographic polissacaru00ecdeo cross-linked to provide a substantially sterile chromatographic column and to provide a membrane polissacaru00ecdeo substantially sterile chromatographic matrix porous polissacaru00ecdeo cross-linked chromatographic column. The use of a chromatographic matrix.

机译：制备交联的基质色谱波利沙卡尔交联色谱法的方法，以提供一种基本无菌的色谱柱，并提供一种膜状波利斯卡丁基本无菌的色谱法基质多孔波律沙卡尔交联的色谱柱。使用色谱基质。

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Methods of preparing a matrix chromatographic polissacaru00ecdeo cross-linked to provide a substantially sterile chromatographic column and to provide a membrane polissacaru00ecdeo substantially sterile chromatographic matrix porous polissacaru00ecdeo cross-linked chromatographic column. And, use of a chromatographic matrix ".The present invention relates to a method for the preparation of a chromatographic matrix of cross-linked polysaccharide, which comprises providing an aqueous solution of a polysaccharide geleificu00e1vel, in which part of the hydroxyl groups are replaced by groups.They are not susceptible to attack nucleofu00edlico; to provide essentially spherical droplets of the solution of the polysaccharide replaced; to form a gel of polysaccharide solution replaced; and the reticular gel. The invention also includes a chromatographic column packed with a matrix thus prepared, and use of same in, for example, the purification of the protein.

机译：制备交联的基质色谱波利沙卡尔交联色谱法的方法，以提供一种基本无菌的色谱柱，并提供一种膜状波利斯卡丁基本无菌的色谱法基质多孔波律沙卡尔交联的色谱柱。并且，涉及使用色谱基质。它们不易受核键的攻击;不能提供被替换的多糖溶液的基本上球形的液滴;可以形成被替换的多糖溶液的凝胶;以及网状凝胶;本发明还包括一种填充了色谱柱的色谱柱。如此制备的基质，并将其用于例如蛋白质的纯化中。

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公开/公告号BRPI0515586A

专利类型
公开/公告日2008-07-29

原文格式PDF
申请/专利权人 GE HEALTHCARE BIO-SCIENCES AB;
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申请/专利号BR2005PI15586
发明设计人 HANS BERG;MARIA BRONNHOLM;DAVID BUCKLEV;ANDERS HAGVALL;EVA HOLMGREN;HENRIK IHRE;ANDERS LARSSON;DAG LINDSTR÷M;
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申请日2005-09-21
分类号B01J20/30;B01J20/24;B01J20/291;
国家 BR
入库时间 2022-08-21 20:08:45

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