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System and method for quantitative or qualitative measurement of exogenous substances in tissue and other materials using laser-induced fluorescence spectroscopy
System and method for quantitative or qualitative measurement of exogenous substances in tissue and other materials using laser-induced fluorescence spectroscopy
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机译:使用激光诱导荧光光谱法定量或定性测量组织和其他材料中外源性物质的系统和方法
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摘要
Methods and systems for determination of one or more substances within a material are described. A flow of fluorescence-exciting/ablative energy (e.g., laser pulse(s), preferably in the ultraviolet region (e.g. 193-nm)), is directed onto the material to ablate a thin layer of the material using photochemical decomposition. Simultaneously, the laser energy induces fluorescence of the substance(s) within the ablated layer of the material. The fluorescence emitted by the substance(s) is then received by a device, which measures the spectrum of the received fluorescence. The fluorescence spectra are then transmitted to a spectral processing device adapted to determine, on the basis of the fluorescence spectra, whether the substance(s) of interest is/are present in the material and/or the concentration at which the substance(s) of interest is/are present in the material. This process may be repeated for each layer of the material to determine the concentration gradient of the substance(s) of interest in the material.
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