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Methods for the purification and aqueous fiber spinning of spider silks and other structural proteins

机译:蜘蛛丝和其他结构蛋白的纯化和水纤维纺丝的方法

摘要

Methods are described for the purification and spinning of recombinant and non-recombinant proteins. Specifically, the lysis of bacteria and purification of silk proteins occur in a single solution of organic acid. Bacterial proteins are hydrolyzed while the silk protein remains intact. Silk proteins remain soluble as they are concentrated into a aqueous-based mixture for fiber spinning.
机译:描述了纯化和旋转重组和非重组蛋白的方法。具体而言,细菌的裂解和丝蛋白的纯化发生在有机酸的单一溶液中。细菌蛋白水解,而蚕丝蛋白保持完整。丝蛋白在浓缩成水基混合物进行纤维纺丝时仍保持可溶。

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