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Glycerol-3-phosphate phosphatase and glycerol-3-phosphate dehydrogenase from Candida albicans, genes encoding the same, vector and host cell containing the genes, and method for producing glycerol using the host cell
Glycerol-3-phosphate phosphatase and glycerol-3-phosphate dehydrogenase from Candida albicans, genes encoding the same, vector and host cell containing the genes, and method for producing glycerol using the host cell
Provided are a polypeptide having glycerol-3-phosphate dehydrogenase activity and a 80% or more homology to an amino acid sequence as set forth in SEQ ID NO: 1 and a polypeptide having glycerol-3-phosphate phosphatase activity and a 80% or more homology to an amino acid sequence as set forth in SEQ ID NO: 2. Provided is also a method for producing glycerol, which includes: culturing a host cell transformed with a vector containing a polynucleotide including a first polynucleotide encoding an amino acid sequence as set forth in SEQ ID NO: 1 and a second polynucleotide encoding an amino acid sequence as set forth in SEQ ID NO: 2 which are operably linked to a suitable regulatory sequence; and recovering glycerol from the culture.
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机译：提供了具有3-磷酸甘油脱氢酶活性并且与SEQ ID NO：1所示的氨基酸序列具有80％或更高的同源性的多肽，以及具有-磷酸3-甘油磷酸酶活性并且具有80％或更高的多肽。还提供了一种与SEQ ID NO：2所示的氨基酸序列同源的方法。该方法还包括：培养用用含有多核苷酸的载体转化的宿主细胞的宿主细胞，所述多核苷酸包含编码所设置的氨基酸序列的第一多核苷酸SEQ ID NO：1中所示的第二个多核苷酸和编码如SEQ ID NO：2中所示的氨基酸序列的第二多核苷酸，所述第二个多核苷酸可操作地连接至合适的调控序列;并从培养物中回收甘油。
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