首页> 外国专利> Drosophila clipped FRT (cFRT) chromosome insensitive to P transposage, generating method thereof, and application thereof

Drosophila clipped FRT (cFRT) chromosome insensitive to P transposage, generating method thereof, and application thereof

机译：果蝇夹住了对 P 转座不敏感的 FRT （cFRT）染色体，其产生方法和应用

展开▼

页面导航

摘要

著录项

相似文献

摘要

A method for generating a Drosophila clipped FRT (cFRT) chromosome is provided, wherein the chromosome is insensitive to a P transposase but remains functional to a yeast site-specific flippase recombinase (FLP). The method includes steps of: (a) exposing a FRT chromosome to the P transposase for occurring a local and imprecise transposition, wherein the FRT chromosome contains a P[FRT] insertion with a selection marker gene, (b) screening the P[FRT] insertion insensitive to the P transposase to obtain screened products, (c) selecting candidate products from the screened products by further examinations, and (d) exposing the candidate products by the P transposase and selecting a desired product by the further examinations to obtain the Drosophila clipped FRT (cFRT) chromosome insensitive to the P transposase but remaining functional to the yeast site-specific flippase recombinase. The cFRT^2L2Rchromosome can be used as the direct target in the direct P-transposon-induced mutagenesis. 展开▼

机译：本发明提供了一种产生果蝇的FRT（cFRT）染色体的方法，其中该染色体对P转座酶不敏感，但对酵母位点特异性的脂肪酶重组酶（FLP）保持功能。该方法包括以下步骤：（a）将FRT染色体暴露于P转座酶以发生局部和不精确的转座，其中FRT染色体包含带有选择标记基因的P [FRT]插入，（b）筛选P [FRT ]对P转座酶不敏感的插入以获得筛选产物，（c）通过进一步检查从筛选产物中选择候选产物，以及（d）通过P转座酶暴露候选产物并通过进一步检查选择所需产物以获得P 果蝇剪切的FRT（cFRT）染色体对P转座酶不敏感，但对酵母位点特异性的flippase重组酶仍然起作用。 cFRT ^{2L2R 染色体可用作直接P-转座子诱导诱变的直接靶标。} 展开▼

著录项

公开/公告号US7344864B2

专利类型

公开/公告日2008-03-18

原文格式PDF

申请/专利权人 TZE-BIN CHOU;
展开▼

申请/专利号US20030690176

发明设计人 TZE-BIN CHOU;
展开▼

申请日2003-10-20

分类号C12P19/34;C12N15/63;

国家 US

入库时间 2022-08-21 20:11:10

相似文献

专利

外文文献

中文文献

1. 应用早熟染色体凝集预测肿瘤细胞辐射敏感性的方法 [P] . 中国专利： CN101126719A . 2008-02-20

2. 一种果蝇唾腺染色体永久装片的制作方法 [P] . 中国专利： CN114018675A . 2022-02-08

3. Drosophila clipped FRT (CFRT) chromosome insensitive to P transposage, generating method thereof, and application thereof [P] . 美国专利： US2004064262A1 . 2004-04-01

机译：对P转座不敏感的果蝇截短的FRT（CFRT）染色体及其产生方法和应用

4. Drosophila clipped FRT (cFRT) chromosome insensitive to P transposase, generating method thereof, and application thereof [P] . 美国专利： US7189542B2 . 2007-03-13

机译：对 P 转座酶不敏感的果蝇剪裁的FRT（cFRT）染色体，其产生方法和应用

5. Drosophila clipped FRT (cFRT) chromosome insensitive to P transposase, generating method thereof, and application thereof [P] . 美国专利： US6962804B2 . 2005-11-08

机译：对P转座酶不敏感的果蝇剪裁的FRT（cFRT）染色体及其产生方法和应用

1. A New Generating Method and Its Application [J] . 宋中民系统工程与电子技术（英文版） . 2003,第002期

2. A Simple Method to Generate Chromosomal Mutations in Lactobacillus plantarum Strain TF103 to Eliminate Undesired Fermentation Products [C] . SlQING LlU, US Department of Energys Office of the Biomass Program, US Department of Agriculture, Symposium on Biotechnology for Fuels and Chemicals . 2006

3. Methods for Generating High-Fidelity Trace Chemical Residue Reflectance Signatures for Active Spectroscopy Classification Applications [D] . Murphy, Cara Patrice. 2021

4. Analysis of novel alleles of brother of tout-velu the Drosophila ortholog of human EXTL3 using a newly developed FRT42D ovoD chromosome [O] . Ernesto Lujan, Douglas J. Bornemann, Carmen Rottig, -1

5. An Efficient Method to Generate Chromosomal Rearrangements by Targeted DNA Double-Strand Breaks in Drosophila melanogaster [O] . Egli, Dieter, Hafen, Ernst, Schaffner, Walter 2004

1. A New Method to Generate the Complete Set of Unique Signals for Application in High Consequence System [J] . 谈顺毅 ,陈文元 ,张卫平 . 上海交通大学学报：英文版 . 2007,第2期

2. 污泥滤干技术瓶颈的解决方法和“以污治污”方法在环保综合治理中的应用及产生的巨大社会经济效益 [C] . 肖克绳 . 第二届中国绿色财富论坛暨科技创新与可持续发展研讨会 . 2006

3. 决定果蝇对吸入麻醉药敏感性的基因的其常染色体的定位 [A] . 金传刚 . 2000