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Method For Identifying Agents Which Modulate GtpPase Activity Involved in Insulin-Stimulated Glut4 Translocation

机译:鉴定与胰岛素刺激的Glut4易位有关的调节GtpPase活性的药物的方法

摘要

The present invention is a method for identifying agents that modulate the GTPase activity of AS160. In the instant assay, AS160 or the GAP domain thereof is contacted with a test agent, in the presence of GTP-bound Rab (2A, 8A, 8B, 10, or 14), and the AS160 GAP domain-mediated hydrolysis of GTP to GDP is monitored.
机译:本发明是用于鉴定调节AS160的GTP酶活性的试剂的方法。在本试验中,在结合有GTP的Rab(2A,8A,8B,10或14)和AS160 GAP域介导的GTP水解为GDP受到监控。

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