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In the oxazoline derivative of production mannered null

机译:在恶唑啉衍生物的生产中提倡无效

摘要

PROBLEM TO BE SOLVED: To provide a method for easily mass-producing a β-1,6-bound N- acetyllactosamine oligosaccharide at a low cost.;SOLUTION: The β-1,6-bound N-acetyllactosamine oligosaccharide of the formula (1): Galβ-1-4(GlcNAcβ1-6Galβ1-4)nGlcNAc (wherein, Gal is a galactose residue; GlcAc is an N-acetylglucosamine residue; and (n) is 1-4) where N- acetyllactosamine is repeatedly bound via β-1,6 linkages is obtained by converting chitinase into an oxazoline derivative of N-acetyllactosamine under alkaline conditions to effect a transfer reaction into N-acetyllactosamine as the hydrolyzate of the oxazoline derivative. Further, β-D-galactosidase is reacted to the β-1,6-bound N-acetyllactosamine oligosaccharide to cleave the galactose residue on the non-reduced terminal, thus obtaining another type of β-1,6-bound N-acetyllactosamine oligosaccharide bearing N-acetylglucosamine on the non- reduced terminal.;COPYRIGHT: (C)2003,JPO
机译:解决的问题:提供一种容易以低成本容易地大量生产β-1,6-结合的N-乙酰基乳糖胺低聚糖的方法。解决方案:该β--1,6-结合的N-乙酰基乳糖胺低聚糖式(1):Galβ-1-4(GlcNAcβ1-6Galβ1-4)nSubGlcNAc(其中,Gal是半乳糖残基; GlcAc是N-乙酰基葡糖胺残基;和( n)是1-4),其中通过在碱性条件下将几丁质酶转化为N-乙酰基乳糖胺的恶唑啉衍生物,以实现作为水解产物的N-乙酰基乳糖胺的转移反应,从而通过β-1,6键重复地结合N-乙酰基乳糖胺。恶唑啉衍生物。此外,β-D-半乳糖苷酶与β-1,6-结合的N-乙酰基乳糖胺寡糖反应,以切割非还原末端上的半乳糖残基,从而获得另一种β-1,6-结合的N -N-乙酰氨基葡糖在非还原末端带有N-乙酰氨基葡糖的寡糖。;版权所有:(C)2003,JPO

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