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for enzymes that have the ability of linear alpha 1,4 - glucane in plants, fungi and microorganisms to synthesieren, chemistry dna sequences

机译：用于植物，真菌和微生物中具有线性α1,4-葡聚糖能力的酶合成化学dna序列

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摘要

The invention relates to DNA sequences coding for proteins having the enzymatic activity of an amylosucrase which allows the synthesis of linear alpha-1,4 glucans from the substrate sucrose by bacteria, fungi and plants or in cell-free systems. The invention furthermore describes plasmids and bacteria containing these DNA sequences as well as processes for the production of plants and microorganisms capable of intracellularly or extracellularly expressing a polypeptide having amylosucrase activity. The invention furthermore relates to the production of pure fructose using proteins exhibiting the enzymatic activity of amylosucrase.

机译：本发明涉及编码具有淀粉蔗糖酶的酶活性的蛋白质的DNA序列，其允许细菌，真菌和植物或在无细胞系统中从底物蔗糖合成线性α-1，4葡聚糖。本发明还描述了含有这些DNA序列的质粒和细菌，以及生产能够在细胞内或细胞外表达具有淀粉糖核酸酶活性的多肽的植物和微生物的方法。此外，本发明涉及使用显示出淀粉淀粉酶的酶促活性的蛋白质来生产纯果糖。

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公开/公告号DE69535543D1

专利类型
公开/公告日2007-09-06

原文格式PDF
申请/专利权人 BAYER BIOSCIENCE GMBH;
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申请/专利号DE19956035543T
发明设计人 KOSSMANN JENS;BUETTCHER VOLKER;WELSH THOMAS;
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申请日1995-05-18
分类号A01H5;C12N15/54;C07H21/04;C08B30/20;C12N1/15;C12N1/21;C12N5/10;C12N9/10;C12N9/30;C12N15/09;C12N15/74;C12N15/82;C12P19/18;C12Q1/68;
国家 DE
入库时间 2022-08-21 20:27:30

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