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Detection of mycobacterium tuberculosis by replication of nucleic acid sequences

机译：通过复制核酸序列检测结核分枝杆菌

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摘要

A method, composition and kit for synthesizing multiple copies of a target nucleic acid sequence autocatalytically under conditions of substantially constant temperature, ionic strength, and pH are provided in which multiple RNA copies of the target sequence autocatalytically generate additional copies using a mixture of blocked and unblocked primers and/or promoter-primers to initiate DNA and RNA synthesis, preferably with reduced non-specific product formation. The invention is useful for generating copies of a nucleic acid target sequence for purposes that include assays to quantitate specific nucleic acid sequences in clinical, environmental, forensic and similar samples, cloning and generating probes.

机译：提供了一种在基本上恒定的温度，离子强度和pH的条件下自动催化合成靶核酸序列的多个拷贝的方法，组合物和试剂盒，其中使用封闭的和封闭的混合物，靶序列的多个RNA拷贝自动催化产生另外的拷贝。非封闭的引物和/或启动子引物，以启动DNA和RNA合成，最好减少非特异性产物的形成。本发明可用于产生核酸靶序列的拷贝，以用于包括定量定量临床，环境，法医和类似样品中的特定核酸序列的测定，克隆和产生探针的目的。

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公开/公告号DE69334092D1

专利类型
公开/公告日2007-01-11

原文格式PDF
申请/专利权人 GEN-PROBE INC.;
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申请/专利号DE19936034092T
发明设计人 MCALLISTER DIANE L.;HAMMOND PHILIP W.;YEASING Y. YANG;MCDONOUGH SHERROL;KACIAN DANIEL;DATTAGUPTA NANIBHUSHAN;RYDER THOMAS B.;
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申请日1993-08-04
分类号C12N15/09;C12Q1/68;C07H;C07H21/04;C07K14/35;C12N15/10;C12P19/34;C12Q1/70;C12R1/32;C12R1/36;G01N33/53;G01N33/566;
国家 DE
入库时间 2022-08-21 20:27:30

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