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PROCESS FOR THE PREPARATION OF L-AMINO ACIDS USING TREES FROM THE FAMILY OF THE ENTEROBACTERIACEA CONTAINING ATTACHED ASPA GENE

机译：利用含有附加的ASPA基因的肠杆菌科植物中的树木制备L-氨基酸的方法

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Preparing (M) L-amino acids, e.g. L-threonine comprising fermenting microorganisms of Enterobacteriaceae family in which one or more of genes malE, phoA, phoB, phoR, phoE, phnC to phnG, phnJ to phnP, pykF, pfkB, eda, talB, rpiB, zwf, mopA, pstA to pstC, pstS, ugpA, ugpE, ugpC, ugpQ, dnaK, dnaJ, clpB, rpoE, rseA, rseC, htpG, sodA, ompF, ompC, sucA to sucD, gltA and sdhB, or the sequence coding for it, is attenuated, is new. (M) comprises fermenting microorganisms of the Enterobacteriaceae family which produce the desired L-amino acid and in which one or more of the genes malE (periplasmic binding protein for maltose transport), phoA (alkaline phosphatase), phoB (regulatory protein of the pho regulon), phoR (sensor protein of the pho regulon), phoE (pore protein E of the outer cell membrane), phnC (ATP-binding protein of the alkyl phosphonate transport system), phnD (substrate-binding protein of the alkyl phosphonate transport system), phnE (permease protein of the alkyl phosphonate transport system), phnF (putative regulatory protein), phnG, phnJ, phnM and phnP (membrane-bound subunit of the carbon-phosphorus lyase complex), phnK, phnL and phnN (ATP-binding proteins of the phosphonate transporter), phnO (putative regulator of the carbon-phosphorus lyase complex), pykF (fructose-stimulated pyruvate kinase I), pfkB (6-phosphofructokinase isoenzyme 2), eda (2-keto-3-deoxygluconate 6-phosphate aldolase and 2-keto-4-hydroxyglutarate aldolase (Entner-Doudoroff aldolase)), talB (transaldolase B), rpiB (ribose 5-phosphate isomerase B), zwf (glucose 6-phosphate 1-dehydrogenase), mopA (Chaperone GroEL, heat shock protein Hsp60), pstA (permease protein of the high-affinity phosphate transport system), pstB (ATP binding component of the high-affinity phosphate transport system), pstC (permease protein of the high-affinity phosphonate transport system), pstS (periplasmic phosphate-binding protein of the high-affinity phosphate transport system), ugpA and ugpE (permease protein of the sn-glycerol 3-phosphate transport system), ugpC (ATP-binding component of the sn-glycerol 3-phosphate transport system), ugpQ (glycerol phosphodiester phosphodiesterase), dnaK (autoregulated heat shock protein Hsp70), dnaJ (Chaperone, heat shock protein), clpB (heat shock protein f84.1), rpoE (=s-E subunit of RNA polymerase), rseA (membrane protein with anti-=sE activity), rseC (regulatory protein of the =sE factor), htpG (Chaperone, heat shock protein c62.5), sodA (superoxide dismutase), ompF (outer membrane protein F), ompC (outer membrane protein C), sucA (decarboxylase subunit of 2-ketoglutarate dehydrogenase), sucB (dihydrolipoyltranssuccinase subunit of 2-ketoglutarate dehydrogenase), sucC ( beta -subunit of succinyl-CoA synthetase), sucD ( alpha -subunit of succinyl-CoA synthetase), gltA (citrate synthase) and sdhB (iron-sulfur protein subunit of succinate dehydrogenase), or the nucleotide sequence which codes for this, is or are attenuated, in particular eliminated, concentrating the desired L-amino acid in the medium or in the cells of the microorganisms, and isolating the desired L-amino acid, constituents of the fermentation broth and/or the biomass in its entirety or its portions (greater than 0 to 100%) optionally remaining in the product.

机译：制备（M）L-氨基酸，例如包含肠杆菌科发酵微生物的L-苏氨酸，其中malE，phoA，phoB，phoR，phoE，phnC至phnG，phnJ至phnP，pykF，pfkB，eda，talB，rpiB，zwf，mopA，pstA至pstA pstC，pstS，ugpA，ugpE，ugpC，ugpQ，dnaK，dnaJ，clpB，rpoE，rseA，rseC，htpG，sodA，ompF，ompC，sucA转换为sucD，gltA和sdhB，或为其编码的序列，是新的。（M）包括肠杆菌科的发酵微生物，其产生所需的L-氨基酸，并且其中一个或多个基因malE（麦芽糖转运的周质结合蛋白），phoA（碱性磷酸酶），phoB（pho的调节蛋白） regulon），phoR（pho regulon的传感蛋白），phoE（外细胞膜的孔蛋白E），phnC（烷基膦酸酯转运系统的ATP结合蛋白），phnD（烷基膦酸酯转运的底物结合蛋白）系统），phnE（烷基膦酸酯转运系统的渗透酶蛋白），phnF（假定的调节蛋白），phnG，phnJ，phnM和phnP（碳-磷裂解酶复合物的膜结合亚基），phnK，phnL和phnN（ATP转运蛋白的结合蛋白），phnO（碳-磷裂解酶复合物的假定调节剂），pykF（果糖刺激的丙酮酸激酶I），pfkB（6-磷酸果糖激酶同工酶2），eda（2-酮-3-脱氧葡萄糖酸酯） 6-磷酸醛缩酶和2- keto-4-hydroxyglutarate aldolase（Entner-Doudoroff aldolase）），talB（transaldolase B），rpiB（核糖5磷酸异构酶B），zwf（葡萄糖6磷酸1-脱氢酶），mopA（伴侣GroEL，热休克蛋白Hsp60 ），pstA（高亲和力磷酸盐转运系统的渗透酶蛋白），pstB（高亲和力磷酸盐转运系统的ATP结合成分），pstC（高亲和力磷酸盐转运系统的渗透酶蛋白），pstS（周质磷酸盐-高亲和力磷酸盐转运系统的结合蛋白），ugpA和ugpE（3-磷酸甘油甘油转运系统的渗透酶蛋白），ugpC（3-磷酸甘油甘油转运系统的ATP结合成分），ugpQ（甘油磷酸二酯磷酸二酯酶），dnaK（自动调节的热休克蛋白Hsp70），dnaJ（分子伴侣，热休克蛋白），clpB（热休克蛋白f84.1），rpoE（RNA聚合酶的 sE亚基），rseA（具有抗性的膜蛋白- = sE活性），rseC（ = sE因子的调节蛋白），htpG（伴侣蛋白，热休克蛋白c62.5），sodA（超氧化物歧化酶），ompF（外膜蛋白F），ompC（外膜蛋白C），sucA（2-酮戊二酸脱氢酶的脱羧酶亚基），sucB（二氢脂酰转琥珀酸酶亚基） 2-酮戊二酸脱氢酶），sucC（琥珀酰-CoA合成酶的β-亚基），sucD（琥珀酰-CoA合成酶的α-亚基），gltA（柠檬酸合酶）和sdhB（琥珀酸脱氢酶的铁-硫蛋白亚基）或编码或编码的核苷酸序列被或被减毒，特别是被消除，在微生物的培养基或细胞中浓缩所需的L-氨基酸，并分离出所需的L-氨基酸，发酵液和/或生物质的全部或部分（大于0至100％）（可选）保留在产品中。

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公开/公告号DE60210620T2

专利类型
公开/公告日2007-04-05

原文格式PDF
申请/专利权人 DEGUSSA AG;
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申请/专利号DE20026010620T
发明设计人
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申请日2002-07-03
分类号C12P13/08;C12P13/12;C12P13/14;C12P13/06;C12P13/10;C12P13/20;C12P13/22;C12P13/24;
国家 DE
入库时间 2022-08-21 20:27:59

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